Evaluation of Solid Free-Form Fabrication-Based Scaffolds Seeded with Osteoblasts and Human Umbilical Vein Endothelial Cells for Use In Vivo Osteogenesis
- Evaluation of Solid Free-Form Fabrication-Based Scaffolds Seeded with Osteoblasts and Human Umbilical Vein Endothelial Cells for Use In Vivo Osteogenesis
- 김종영; Jin Guang-zhen; 박인수; 김정남; 천소영; 박의균; 김신윤; James Yoo; 김상헌; 이종원; 조동우
- scaffold; osteoblast; osteogenesis; SFF fabrication
- Issue Date
- Tissue engineering. Part A.
- VOL 16, NO 7, 2229-2236
- We investigated the feasibility of using solid free-form fabrication (SFF)-based scaffolds seeded with osteoblasts,
derived from human adipose-derived stem cells, and human umbilical vein endothelial cells (HUVECs) to
enhance osteogenesis. To accomplish this goal, SFF-based polycaprolactone/poly-lactic-co-glycolic acid/tricalcium
phosphate scaffolds were fabricated using a multihead deposition system, which is one of SFF techniques.
The blended polycaprolactone/poly-lactic-co-glycolic acid/TCP scaffolds were seeded with human
osteoblasts and HUVECs and implanted into calvaria defects in rats. At 8 and 12 weeks after implantation,
microcomputed tomography, real-time polymerase chain reaction, and histological assays (hematoxylin and
eosin staining and Alizarin red staining) were conducted to determine the effects of SFF-based scaffolds on
osteogenic potential. In vivo experiments indicated that the osteoblast-only and osteoblast-HUVEC group produced
bone formation. Additionally, scaffolds in the osteoblast-HUVEC group had the largest area of new bone
tissue. Therefore, we demonstrated through microcomputed tomography and histological assays that scaffolds
seeded with both human osteoblasts and HUVECs were superior to other groups for effective bone formation.
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