Autoradiographic Verification of Transdermal Penetration of Oleic Acid-conjugated Peptide Nanosomes
- Autoradiographic Verification of Transdermal Penetration of Oleic Acid-conjugated Peptide Nanosomes
- 이경은; 정민교; 엄재훈; 정세희; 하권수; 박정해; 이진성; 한성식; 최면
- Autoradiography; Oleic acid-conjugated peptide; Transdermal penetration
- Issue Date
- Korean Journal of Microscopy
- VOL 40, NO 4, 185-191
- Short peptides are potentially effective materials as cosmeceuticals, but their delivery across the skin can be problematic
due to the ionic nature of peptides and the structure of the skin. For short peptide to be utilized as cosmeceuticals, its
ability to penetrate the skin must be altered. In this study, we conjugated the widely used procollagen type I signal
peptide, KTTKS, with oleic acid to improve the lipophilic properties of the peptide, and used the oleic acid-conjugated
peptides to construct cosmeceutical nanosomes. Then we examined the penetration of cosmeceutical nanosomes prepared
from isotope-labeled peptide into the skin after transdermal application using autoradiography. Because of its hydrophilic
property of penta-peptide, the penta-peptide itself was not able to be penetrated through the stratum corneum of the skin.
In contrast, nanosomes made from olecic acid conjugated penta-peptide were able to be penetrated through the stratum
corneum effectively. Autoradiography showed the precise penetration points to dermal layer, demonstrating the appropriateness
of this method for clarifying the mechanism of penetration of transdermal delivery systems.
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