Adhesion and proliferation of chondrocyte in 3D environment of RGD-grafted F127 hydrogel

Abstract

The F127 derivatives hydrogels were successfully synthesized and used for 3D culture of chondrocytes. The cell viability was low, due to the cytotoxicity with higher content of APS/TEMED used during the crossliking of hydrogel. For the improvement of cell viability in hydrogel, the pH of FM gels was adjusted using NaOH and as a result the cell viability increased substantially. F127-based hydrogels grafted RGD peptide sequence are expected as an environment for 3D cell culture.