A novel GC-MS method in urinary estrogen analysis from postmenopausal women with osteoporosis

Title
A novel GC-MS method in urinary estrogen analysis from postmenopausal women with osteoporosis
Authors
문주연김광준문명희정봉철최만호
Keywords
steroid porfiling; ethoxycarbonylation; estrogen; osteoporosis; menopausal women; hormones
Issue Date
2011-08
Publisher
Journal of lipid research
Citation
VOL 52, NO 8, 1595-1603
Abstract
Estrogen metabolites play important roles in the development of female-related disorders and homeostasis of the bone. To improve detectability, a validated gas chromatography- mass spectrometry (GC-MS) method was conducted with two-phase extractive ethoxycarbonlyation (EOC) and subsequent pentafl uoropropionyl (PFP) derivatization was introduced. The resulting samples were separated through a high-temperature MXT-1 column within an 8 min run and were detected in the selected ion monitoring (SIM) mode. The optimized analytical conditions led to good separation with a symmetric peak shape for 19 estrogens as their EOC-PFP derivatives. The limit of quantifi cation (LOQ) was from 0.02 to ~ 0.1 ng/ml for most estrogens analyzed, except for 2-hydroxyestriol (0.5 ng/ml). The devised method was found to be linear ( r2 > 0.995) in the range from the LOQ to 40 ng/ml, whereas the precision (% CV) and accuracy (% bias) ranged from 1.4 to 10.5% and from 91.4 to 108.5%, respectively. The good sensitivity and selectivity of this method even allowed quantifi cation of the estrogen metabolites in urine samples obtained from the postmenopausal female patients with osteoporosis. The present technique can be useful for clinical diagnosis as well as to better understand the pathogenesis of estrogen-related disorders in low-level quantifi cation.
URI
https://pubs.kist.re.kr/handle/201004/40067
ISSN
0022-2275
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KIST Publication > Article
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