Adhesion Behavior of Chondrocyte and Osteoblast on Biodegradable Surface-Modified PLLA Films and Scaffolds

Abstract

Surface-modified poly(L-lactic acid) (PLLA) films and scaffolds that were treated with plasma discharge in oxygen gas and subsequently were subjected to in situ grafting of acrylic acid (AA) in order to increase the cell compatibility. The surface of AA-grafted PLLA was converted to hydroxyapatite (HA)-deposited PLLA in stimulated body fluid (SBF). After the samples were immersed in phosphate-buffered saline (PBS), fetal bovine serum (FBS), normal saline, and cell medium, the water contact angles were significantly reduced on the surface of HA-deposited PLLA. Chondrocyteand osteoblast appeared a higher attachment and significant cell proliferation on HA-deposited surfaces and in particular, it was confirmed that chondrocyte was considerably influenced by HA. However, osteoblast showed better cell proliferation on the surfaces immersed in FBS and cell medium as well as HA-deposited surface. In addition, the cell proliferation in 3D scaffolds was much higher than that on film type, irrespective of chondrocyte and osteoblast. Therefore, such surface-modified PLLAs are expected to be useful as organic-inorganic hybrid scaffolds in the regeneration of cartilage and bone.