Edible seaweed, Eisenia bicyclis, protects retinal ganglion cells death caused by oxidative stress
- Edible seaweed, Eisenia bicyclis, protects retinal ganglion cells death caused by oxidative stress
- 김경아; 김상민; 강석우; 전상일; 엄병헌; 정상훈
- Eisenia bicyclis; Neuroprotection; NMDA; RGC-5; ROS
- Issue Date
- Marine biotechnology
- VOL 14, NO 4, 383-395
- The purpose of the present study was to determine
whether edible seaweed, Eisenia bicyclis, is effective in blunting
the negative influence of N-methyl-D-aspartate (NMDA)
on rat retinas and of oxidative stress-induced transformed
retinal ganglion cell (RGC-5 cell line) death. The ethanol
extract of E. bicyclis (EEEB) significantly attenuated the
negative insult of L-buthionine-(S,R)-sulfoximine plus glutamate
on RGC-5 cells. Treatment of the RGC-5 cells with
EEEB reduced the reactive oxygen species and recovered
the reduced glutathione level caused by various radical species
such as H2O2, OH·, or O2·−. Moreover, EEEB inhibited lipid
peroxidation on rat brain homogenates caused by sodium
nitroprusside. Applying NMDA to the retina affected the
thickness of the inner plexiform layer (IPL) and terminal
deoxynucleotidyl transferase-mediated dUTP nick-end labeling
(TUNEL) produced a positive effect on ganglion cells.
Importantly, EEEB protected the thinning of IPL and increased
TUNEL positive cells in the ganglion cell layer
(GCL). Five phlorotannin derivatives were isolated using
chromatographic methods and liquid chromatography-mass
spectroscopy analysis which has been known as an antioxidant.
In conclusion, EEEB has a neuroprotective effect in vitro
and in vivo. Furthermore, the major constituents of this
extract, phlorotannins, could possibly be active compounds
due to their antioxidative potency.
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