An Immunoassay Utilizing DNA-Coated Cage Protein As a Signal Generator

Title
An Immunoassay Utilizing DNA-Coated Cage Protein As a Signal Generator
Authors
Hoa Thi Le이태민김병수안형준김태우신미란안대로
Keywords
Cage protein; Immunoassay; Fluorescence intensity; OLISA; Alpha-fetoprotein (AFP)
Issue Date
2014-08
Publisher
Bulletin of the Korean Chemical Society
Citation
VOL 35, NO 8, 2559-2562
Abstract
In summary, we have prepared the DNA-coated cage protein and employed it in an immunoassay system, called CP-OLISA utilizing the cage protein for conjugating dAb with multiple signal generating DNA strands. Compared with the previous methods such as ELISA and OLISA, the CP-OLISA showed improved sensitivity in quantitation of AFP in buffer solution. In addition, the CP-OLISA showed acceptable levels of accuracy and reproducibility that are required for practical applications. We therefore expect that the protein-OLISA presented in this study can be useful for clinical diagnosis as well as research applications.
URI
https://pubs.kist.re.kr/handle/201004/48734
ISSN
02532964
Appears in Collections:
KIST Publication > Article
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