Curcumin Induces Apoptotic Cell Death through Oct4 Inhibition and GSK-3β Activation in NCCIT Cells
- Curcumin Induces Apoptotic Cell Death through Oct4 Inhibition and GSK-3β Activation in NCCIT Cells
- 윤지호; 박영균; 이경미; 김정호; 노주원
- OCT4; β-catenin; NCCIT; Curcumin
- Issue Date
- International Conference on
the 19th Annual Meeting of
Korean Society of Cancer Prevention
- Octamer-binding transcription factor (Oct4) is a key regulator of pluripotent cell maintenance, including embryonic stem cells. However, increasing evidence has suggested that Oct4 is also expressed in cancer stem cells (CSCs) and is associated with tumor progression and chemoresistance. Curcumin (CUR) is a widely used cancer chemopreventive agent, and it has been used to treat several diseases including various cancers. In this study, we investigated whether CUR inhibits Oct4 in NCCIT human embryonic carcinoma cells, which express high levels of endogenous Oct4. CUR significantly inhibited Oct4 activity and mRNA and protein levels. Interestingly, we found that CUR treatment increased apoptotic cell death, as confirmed by sub-G0/G1 contents, cleaved caspases, and proapoptotic protein levels and a series of loss-of-function experiments using Oct4 siRNA. Furthermore, CUR induced marked GSK-3β activation, resulting in an increase in apoptosis. These data suggest that CUR induces apoptotic cell death through Oct4 inhibition and GSK-3β activation. Thus, CUR may be a useful cancer chemopreventive agent to suppress tumor progression or to improve chemoresistance by eliminating CSCs.
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