Monitoring Brain Activity with Protein Voltage and Calcium Sensors

Title
Monitoring Brain Activity with Protein Voltage and Calcium Sensors
Authors
Douglas A. StoraceOliver BraubachLei Jin래리코헨Uhna Sung
Issue Date
2015-05
Publisher
Scientific Reports
Citation
VOL 5, NO 10212
Abstract
Understanding the roles of different cell types in the behaviors generated by neural circuits requires protein indicators that report neural activity with high spatio-temporal resolution. Genetically encoded fluorescent protein (FP) voltage sensors, which optically report the electrical activity in distinct cell populations, are, in principle, ideal candidates. Here we demonstrate that the FP voltage sensor ArcLight reports odor-evoked electrical activity in the in vivo mammalian olfactory bulb in single trials using both wide-field and 2-photon imaging. ArcLight resolved fast odorantresponses in individual glomeruli, and distributed odorant responses across a population of glomeruli. Comparisons between ArcLight and the protein calcium sensors GCaMP3 and GCaMP6f revealed that ArcLight had faster temporal kinetics that more clearly distinguished activity elicited by individual odorant inspirations. In contrast, the signals from both GCaMPs were a saturating integral of activity that returned relatively slowly to the baseline. ArcLight enables optical electrophysiology of mammalian neuronal population activity in vivo.
URI
https://pubs.kist.re.kr/handle/201004/49904
ISSN
20452322
Appears in Collections:
KIST Publication > Article
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