Early growth response 1 (Egr-1) directly regulates GABAA receptor α2, α4, and θ subunits in the hippocampus

Title
Early growth response 1 (Egr-1) directly regulates GABAA receptor α2, α4, and θ subunits in the hippocampus
Authors
모지원김종현이동민선웅이현우김현
Keywords
Egr-1; GABAA; receptor,
Issue Date
2015-05
Publisher
Journal of neurochemistry
Citation
VOL 133, 489-500
Abstract
The homeostatic regulation of neuronal activity in glutamatergic and GABAergic synapses is critical for neural circuit development and synaptic plasticity. The induced expression of the transcription factor early growth response 1 (Egr-1) in neurons is tightly associated with many forms of neuronal activity, but the underlying target genes in the brain remained to be elucidated. This study uses a quantitative real-time PCRapproach, in combination with in vivo chromatin immunoprecipitation, and reveals that GABAA receptor subunit, GABRA2 (a2), GABRA4 (a4), and GABRQ (h) genes, are transcriptional targets of Egr-1. Transfection of a construct that overexpresses Egr-1 in neuroblastoma (Neuro2A) cells up-regulates the a2, a4, and h subunits. Given that Egr-1 knockout mice display less GABRA2, GABRA4, and GRBRQ mRNA in the hippocampus, and that Egr-1 directly binds to their promoters and induces mRNA expression, the present findings support a role for Egr-1 as a major regulator for altered GABAA receptor composition in homeostatic plasticity, in a glutamatergic activity-dependent manner
URI
https://pubs.kist.re.kr/handle/201004/49977
ISSN
00223042
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KIST Publication > Article
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