Growth factors-loaded stents modified with hyaluronic acid andheparin for induction of rapid and tight re-endothelialization
- Growth factors-loaded stents modified with hyaluronic acid andheparin for induction of rapid and tight re-endothelialization
- 최동훈; 강성남; 김성민; Samy Olivier Gobaa; Bang Ju Park; Ik Hwan Kim; 정윤기; 한동근
- Re-endothelialization; Stent; Hyaluronic acid; Heparin; Growth factor; Dopamine
- Issue Date
- Colloids and surfaces. B, Biointerfaces
- VOL 141, 602-610
- Rapid re-endothelialization of damaged vessel lining efficiently prevents restenosis and thrombosis and restores original vascular functions. In this study, we designed a novel metallic stent with a heparin-modified surface and used different methods, including 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and divinyl sulfone (DVS), to load growth factors. First we loaded heparin into a dopamine-conjugated hyaluronic acid (HA) coating to serve as a growth factor reservoir. In a second step, we took advantage of the heparin-binding domain of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) to gain advanced re-endothelialization capabilities. We demonstrated that DVS technique offered higher amount of growth factor loading. In vitro assessment also showed better capillary-like structure formation and localized gap junctions when DVS coating was employed. This study suggested that growth factor loaded stent modified by HA and heparin provided the advantage to rapid and tight restoration of endothelium.
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