Cathepsin B-Specific Metabolic Precursor for In Vivo Tumor-Specific Fluorescence Imaging
- Cathepsin B-Specific Metabolic Precursor for In Vivo Tumor-Specific Fluorescence Imaging
- 권익찬; 김광명; 류주희; 이상민; 윤홍열; 심만규; 구희범; 박재형; 김종호; 이슬기; Martin G. Pomper
- click chemistry; drug delivery; imaging agents; metabolic glycoengineering; tumor targeting
- Issue Date
- Angewandte Chemie international edition
- VOL 55, NO 47-14703
- Recently, metabolic glycoengineering with bioorthogonal click reactions has focused on improving the tumor targeting efficiency of nanoparticles as delivery vehicles for anticancer drugs or imaging agents. It is the key technique for developing tumor-specific metabolic precursors that can generate unnatural glycans on the tumor-cell surface. A cathepsin B-specific cleavable substrate (KGRR) conjugated with triacetylated N-azidoacetyl-d-mannosamine (RR-S-Ac3ManNAz) was developed to enable tumor cells to generate unnatural glycans that contain azide groups. The generation of azide groups on the tumor cell surface was exogenously and specifically controlled by the amount of RR-S-Ac3ManNAz that was fed to target tumor cells. Moreover, unnatural glycans on the tumor cell surface were conjugated with near infrared fluorescence (NIRF) dye-labeled molecules by a bioorthogonal click reaction in cell cultures and in tumor-bearing mice. Therefore, our RR-S-Ac3ManNAz is promising for research in tumor-specific imaging or drug delivery.
- Appears in Collections:
- KIST Publication > Article
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.