Analysis of glycerol with isolation of endogenous interferences using "dilute and shoot”strategy and high-resolution mass spectrometry in human urine for antidoping testing
- Analysis of glycerol with isolation of endogenous interferences using "dilute and shoot”strategy and high-resolution mass spectrometry in human urine for antidoping testing
- 권오승; 이강미; 김호준; 이재익; 손정현; 박주형; 김기훈; 민호필; 성창민; 김용석
- Mass spectrometry; Doping control; Dilute and shoot; Orbitrap; Glycerol analysis
- Issue Date
- Mass Spectrometry Letters
- VOL 7, NO 4-115
- Glycerol was identified and isolated from endogenous interferences during analysis of human urine using high-resolution mass spectrometry (HRMS) for doping control. Urinary sample preparation was simple; the samples were diluted with an organic solvent and then analyzed using a liquid chromatography-mass spectrometry (“dilute and shoot” method). Although the interfering ion peaks were observed at the similar retention time of glycerol, the inference could be identified by isolation with HRMS and further investigation. Thus, creatinine was identified as the endogenous interference for glycerol analysis and it also caused ion suppression resulting in the decrease of glycerol signal. This study reports the first identification and efficient isolation of endogenous interferences in human urine for “dilute and shoot” method. The information about ion suppression could be novel to prevent overestimation or a false result for antidoping analysis.
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