Synergistic effects of nanotopography and co-culture with endothelial cells on osteogenesis of mesenchymal stem cells
- Synergistic effects of nanotopography and co-culture with endothelial cells on osteogenesis of mesenchymal stem cells
- 김홍남; Jangho Kim; Ki-Taek Lim; Yeonju Kim; Shambhavi Pandey; Pankaj Garg; Yun-Hoon Choung; Pill-Hoon Choung; Kahp-Yang Suh; Jong Hoon Chung
- Nanotopography; Osteogenesis; Mesenchymal stem cells; Endothelial cells; Co-culture
- Issue Date
- VOL 34, NO 30-7268
- Inspired by the aligned nanostructures and co-existence of vascular cells and stem cells in human cancellous bone, we quantitatively investigated the relative contributions of nanotopography and co-culture with human umbilical endothelial cells (HUVECs) to the osteogenesis of human mesenchymal stem cells (hMSCs). Although both nanotopography and co-culture independently enhanced the osteogenesis of hMSCs, osteogenesis was further enhanced by the two factors in combination, indicating the importance of synergistic cues in stem cell engineering. Interestingly, nanotopography provided a larger relative contribution to the osteogenesis of hMSCs than did co-culture with HUVECs. Furthermore, the osteogenesis of hMSCs was also affected by the density of parallel nanogrooves, exhibiting a maximum at a 1:3 spacing ratio, as defined as the ratio of ridge width to groove width. Analysis of (i) biochemical soluble factors, (ii) hMSC-substrate interaction and (iii) hMSC-HUVEC interaction suggests that (ii) and (iii) play a crucial role in mediating osteogenic phenotypes.
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