Multiplex real-tiem PCR using encoded micro particles for microRNA profiling
- Multiplex real-tiem PCR using encoded micro particles for microRNA profiling
- 김상경; Seungwon Jung
- Encoded particle; Hydrogel; MicroRNA; Multiplex; Real-time PCR
- Issue Date
- functional genomics, Methods molecular biology vol. 1654
- VOL 1654-230
- Multiplex quantitative real-time PCR (qPCR), which measures multiple DNAs in a given sample, has drawn unprecedented attention as a means of verifying the rapidly increasing genetic targets in a single phenotype. We report the detailed procedure of a readily extensible qPCR for multiple microRNA (miRNA) expression analysis using microparticles of primer-immobilized networks as discrete reactors. Individual particles are identified by two-dimensional codes engraved into the particles. It allows high-fidelity signal analysis in the multiplex real-time PCR. During the course of PCR, the amplicons accumulate in the volume of the particles with amplification efficiency over 95%. Tens of miRNAs can be quantitatively profiled in a single PCR reaction of this method.
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