Full metadata record

DC FieldValueLanguage
dc.contributor.author김상경-
dc.contributor.authorSeungwon Jung-
dc.date.accessioned2021-06-09T04:18:34Z-
dc.date.available2021-06-09T04:18:34Z-
dc.date.issued2017-08-
dc.identifier.citationVOL 1654-230-
dc.identifier.issn9781-4939-
dc.identifier.other49434-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/66295-
dc.description.abstractMultiplex quantitative real-time PCR (qPCR), which measures multiple DNAs in a given sample, has drawn unprecedented attention as a means of verifying the rapidly increasing genetic targets in a single phenotype. We report the detailed procedure of a readily extensible qPCR for multiple microRNA (miRNA) expression analysis using microparticles of primer-immobilized networks as discrete reactors. Individual particles are identified by two-dimensional codes engraved into the particles. It allows high-fidelity signal analysis in the multiplex real-time PCR. During the course of PCR, the amplicons accumulate in the volume of the particles with amplification efficiency over 95%. Tens of miRNAs can be quantitatively profiled in a single PCR reaction of this method.-
dc.publisherfunctional genomics, Methods molecular biology vol. 1654-
dc.subjectEncoded particle-
dc.subjectHydrogel-
dc.subjectMicroRNA-
dc.subjectMultiplex-
dc.subjectReal-time PCR-
dc.titleMultiplex real-tiem PCR using encoded micro particles for microRNA profiling-
dc.typeOther-
dc.relation.page221230-
Appears in Collections:
KIST Publication > ETC
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE