Enhancing Fatty Acid Production of Saccharomyces cerevisiae as an Animal Feed Supplement
- Enhancing Fatty Acid Production of Saccharomyces cerevisiae as an Animal Feed Supplement
- 엄영순; 윤성규; 주영철; 강대희; 신상규; 현정은; 우한민; 박철환; 한성옥
- fatty acids production; Saccharomyces cerevisiae; nitrogen optimization; cofactor balancing; animal feed supplement
- Issue Date
- Journal of agricultural and food chemistry
- VOL 65, NO 50-11035
- Saccharomyces cerevisiae is used for edible purposes, such as human food or as an animal feed supplement. Fatty acids are also beneficial as feed supplements, but S. cerevisiae produces small amounts of fatty acids. In this study, we enhanced fatty acid production of S. cerevisiae by overexpressing acetyl-CoA carboxylase, thioesterase, and malic enzyme associated with
fatty acid metabolism. The enhanced strain pAMT showed 2.4-fold higher fatty acids than the wild-type strain. To further increase the fatty acids, various nitrogen sources were analyzed and calcium nitrate was selected as an optimal nitrogen source for fatty acid production. By concentration optimization, 672 mg/L of fatty acids was produced, which was 4.7-fold higher than wildtype strain. These results complement the low level fatty acid production and make it possible to obtain the benefits of fatty acids as an animal feed supplement while, simultaneously, maintaining the advantages of S. cerevisiae.
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