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dc.identifier.citationVOL 67-281-
dc.description.abstractInitial angiogenesis within the first 3&#8239-
dc.description.abstractdays is critical for healing ischemic diseases such as myocardial infarction. Recently, decellularized extracellular matrix (dECM) has been reported to provide tissue-derived ECM components and can be used as a scaffold for cell delivery for angiogenesis in tissue engineering. Decellularization by various detergents such as sodium dodecyl sulfate (SDS) and triton X-100 can remove the cell nuclei in tissue organs. However, this leads to ECM structure denaturation, decreased presence of various ECM proteins and cytokines, and loss of mechanical properties. To overcome these limitations, in this study, we developed a supercritical carbon dioxide and ethanol co-solvent (scCO2-EtOH) decellularization method, which is a detergent-free system that prevents ECM structure disruption and retains various angiogenic proteins in the heart dECM, and tested on rat heart tissues. The heart tissue was placed into the scCO2 reactor and decellularized at 37&#8239-
dc.description.abstract°C and 350&#8239-
dc.description.abstractbar. After scCO2-EtOH treatment, the effects were evaluated by DNA, collagen, and glycosaminoglycan (GAG) quantification and hematoxylin and eosin and immunofluorescence staining to determine the absence of nucleic acids and preservation of heart ECM components. Similar to the native group, the scCO2-EtOH group contained more ECM components such as collagen, GAGs, collagen I, laminin, and fibronectin and angiogenic factors including vascular endothelial growth factor, fibroblast growth factor, and platelet-derived growth factor and others in comparison to the detergent group. In addition, to estimate angiogenesis of the dECM hydrogels, the neutralized dECM solution was injected in a rat subcutaneous layer (n&#8239-
dc.description.abstract6 in each group: collagen, scCO2-EOH, and detergent group), after which the solution naturally formed gelation in the subcutaneous layer. After 3&#8239-
dc.description.abstractdays, the gels were harvested and estimated-
dc.publisherActa Biomaterialia-
dc.titleDecellularized heart ECM hydrogel using supercritical carbon dioxide for improved angiogenesis-
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