Chloro-Functionalized Photo-crosslinking BODIPY for Glutathione Sensing and Subcellular Trafficking
- Chloro-Functionalized Photo-crosslinking BODIPY for Glutathione Sensing and Subcellular Trafficking
- 이준석; 마무눌 하크; 디라제무라레; 홍성철
- 형광; 이미징; 센서; 염료
- Issue Date
- Chembiochem : a European journal of chemical biology
- VOL 19, NO 10-1005
- Glutathione (GSH) is one of major antioxidants inside cells that regulates oxidoreduction homeostasis. Recently, there have been extensive efforts to visualize GSH in live cells, but most
of the probes available today are simple detection sensors and do not provide details of cellular localization. A new fluorescent probe (pcBD2-Cl), which is cell permeable and selectively
reacts with GSH in situ, has been developed. The in situ GSHlabeled probe (pcBD2– GSH) exhibited quenches fluorescence, but subsequent binding to cellular abundant glutathione Stransferase (GST) recovers the fluorescence intensity, which makes it possible to image the GSH– GST complex in live cells. Interactions between probe and GST were confirmed by means of photo-crosslinking under intact live-cell conditions. Interestingly, isomers of chloro-functionalized 4,4-difluoro-4- bora-3a,4a-diaza-s-indacene (BODIPY) compounds behaved very distinctively inside the cells. Following co-staining imaging with MitoTracker and mitochondria fractionation upon lipopolysaccharide-mediated reactive oxygen species induction experiments showed that pcBD2– GSH accumulated in mitochondria. This is the first example of a live-cell imaging probe to visualize translocation of GSH from the cytosol to mitochondria.
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