Proteomic approach to discover organ specific biomarkers for cardiac disease

Proteomic approach to discover organ specific biomarkers for cardiac disease
강민정조예타 마흐무드Hafeza Akter
cardiac disease; biomarkers; mass spectrometry; Mascot
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생화학분자생물학회 (KSBMB)
Abstract for KSBMB 2018 Proteomic approach to discover organ specific biomarkers for cardiac disease Joyeta Mahmud1,2, Hafeza Akter1, Min-Jung Kang1,2* 1 Molecular Recognition Research Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea. 2Division of Bio-medical science and technology, KIST School, Korea University of Science and technology, Seoul 02792, Republic of Korea. Author’s E-mail: Correspondence to: Abstract A large number of patients are seen in the hospitals with symptoms requiring investigation for possible acute myocardial infarction every year. Patients can be distinguished by symptoms, risk factors, and electrocardiogram results but cardiac biomarkers also have a crucial role both diagnostically and prognostically. Our aim is to identify the protein composition & concentration of diseased cardiac cells with healthy cardiac cells also with other diseased organ cells. We are studying different cell lines to detect an organ-specific biomarker for ischaemic heart disease. We have compared the cell lines MKN-1, MKN-45, HFE-145, HEPA-RG, A549, WI38, H9C2 for gastric, liver, lung and cardiac, respectively. In this context, we have evaluated and compared the levels of proteins in the supernatants and media of these cell lines. For our comparative study, samples were analyzed by HPLC-ESI MS/MS and proteins were identified by mass spectrometric data and were searched by Mascot 2.4.0 against the SwissProt human database. We investigated several experimental parameters including reagents for protein solubilization and denaturation, trypsin digestion conditions. After analyzing with mass spectrometry we found some biomarker candidates which were present in cardiac cell line but not in the other organ cell line. Key words: cardiac disease,
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