Full metadata record

DC FieldValueLanguage
dc.contributor.author유영숙-
dc.contributor.author강민정-
dc.contributor.authorHafeza Akter-
dc.contributor.author윤정환-
dc.date.accessioned2021-06-09T04:20:18Z-
dc.date.available2021-06-09T04:20:18Z-
dc.date.issued2018-06-
dc.identifier.citationVOL 41, NO 6-602-
dc.identifier.issn1016-8478-
dc.identifier.other50981-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/67774-
dc.description.abstractGastric cancer is the fifth most common type of malignancy worldwide, and the survival rate of patients with advanced-stage gastric cancer is low, even after receiving chemotherapy. Here, we validated neurotensin receptor 1 (NTSR1) as a potential therapeutic target in gastric cancer. We compared NTSR1 expression levels in sixty different gastric cancer-tissue samples and cells, as well as in other cancer cells (lung, breast, pancreatic, and colon), by assessing NTSR1 expression via semi-quantitative real-time reverse transcription polymerase chain reaction, immunocytochemistry and western blot. Following neurotensin (NT) treatment, we analyzed the expression and activity of matrix metalloproteinase-9 (MMP-9) and further determined the effects on cell migration and invasion via wound-healing and transwell assays. Our results revealed that NTSR1 mRNA levels were higher in gastric cancer tissues than non-cancerous tissues. Both of NTSR1 mRNA levels and expression were higher in gastric cancer cell lines relative to levels observed in other cancer-cell lines. Moreover, NT treatment induced MMP-9 expression and activity in all cancer cell lines, which was significantly decreased following treatment with the NTSR1 antagonist SR48692 or small-interfering RNA targeting NTSR1. Furthermore, NT-mediated metastases was confirmed by observing epithelial-mesenchymal transition markers SNAIL and E-cadherin in gastric cancer cells. NT-mediated invasion and migration of gastric cancer cells were reduced by NTSR1 depletion through the Erk signaling. These findings strongly suggested that NTR1 constitutes a potential therapeutic target for the inhibition of gastric cancer invasion and metastasis.-
dc.publisherMolecules and cells-
dc.subjectgastric cancer-
dc.subjectMMP-9-
dc.subjectneurotensin-
dc.subjectNTSR 1-
dc.titleValidation of Neurotensin Receptor 1 as a therapeutic target for gastric cancer-
dc.typeArticle-
dc.relation.page591602-
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE