Dynamic Light Scattering Analysis to Dissect Intermediates of SNARE-Mediated Membrane Fusion.
- Dynamic Light Scattering Analysis to Dissect Intermediates of SNARE-Mediated Membrane Fusion.
- 양유수; Byoungjae Kong; Dae-Hyuk Kweon
- Issue Date
- Methods in Molecular Biology
- VOL 1860-69
- Dynamic light scattering (DLS) spectroscopy provides rapid information on the size distribution of a large number of particles in a mixture. Vesicle sizes change during the merger of lipid bilayers, and DLS analysis can provide rapid, accurate, and non-perturbative quantification of the size distribution of proteoliposomes in SNARE-dependent membrane fusion. In this chapter, we describe the methodologies and reagents used for DLS spectroscopy in a biochemical and biophysical study of SNARE-mediated membrane fusion.
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