Procaspase activating compound 1 controls tetracycline repressor-regulated gene expression system
- Procaspase activating compound 1 controls tetracycline repressor-regulated gene expression system
- 심태보; 송치만; 이지연; 김남경; 박미리; 오기봉
- anti-induction; antagonist; gene regulation; PAC-1; tetracycline repressor; TetR-regulated system
- Issue Date
- Bioscience reports
- VOL 39, NO 1, BSR20180793
- The tetracycline repressor (TetR)-regulated system is a widely used tool to study gene functions through control of its expression. Various effectors such as tetracycline (Tc) and doxycycline (Dox) quickly induce or shut down gene expression, but reversing gene expression has not been eligible due to long half-lives of such effectors. Here, we found that procaspase activating compound 1 (PAC-1) rapidly reduces transient expression of TetR-regulated green fluorescent protein (GFP) in mammalian cells. Next, we applied PAC-1 to control of expression of transient receptor potential melastatin 7 (TRPM7) protein, whose downstream cellular events can be monitored by cell morphological changes. We observed that PAC-1 quickly reduces TRPM7 expression, consequently affecting cell morphology regulated by TRPM7. The present study demonstrates the first small molecule that efficiently turns off the TetR-regulated gene expression in mammalian cells, thereby precisely regulating the expression level of target gene.
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