ANO1/TMEM16A regulates process maturation in radial glial cells in the developing brain
- ANO1/TMEM16A regulates process maturation in radial glial cells in the developing brain
- 오수진; 황은미; 홍규상; 오우택; 김형섭; 최재혁; 이성훈; 이병준; 장용우; 정주영; 김인범
- Anoctamin 1; TMEM16A; neural stem cell; radial glial cell; cortical development
- Issue Date
- Proceedings of the National Academy of Sciences of the United States of America
- VOL 116, NO 25-12499
- Neural stem cells (NSCs) are primary progenitor cells in the early developmental stage in the brain that initiate a diverse lineage of differentiated neurons and glia. Radial glial cells (RGCs), a type of neural stem cell in the ventricular zone, are essential for nurturing and delivering new immature neurons to the appropriate cortical target layers. Here we report that Anoctamin 1 (ANO1)/TMEM16A, a Ca2+-activated chloride channel, mediates the Ca2+-dependent process extension of RGCs. ANO1 is highly expressed and functionally active in RGCs of the mouse embryonic ventricular zone. Knockdown of ANO1 suppresses RGC process extension and protrusions, whereas ANO1 overexpression stimulates process extension. Among various trophic factors, brain-derived neurotrophic factor (BDNF) activates ANO1, which is required for BDNF-induced process extension in RGCs. More importantly, Ano1-deficient mice exhibited disrupted cortical layers and reduced cortical thickness. We thus conclude that the regulation of RGC process extension by ANO1 contributes to the normal formation of mouse embryonic brain.
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