DNA aptamer immobilized hydroxyapatite for enhancing angiogenesis and bone regeneration
- DNA aptamer immobilized hydroxyapatite for enhancing angiogenesis and bone regeneration
- 전호정; 서영민; 이경우; 손재우; 김준형; 황장선; 최용현; 강호창; 우흥명; 강병재; 최종훈
- hydroxyapatite; aptamer; crosslinker; angiogenesis; bone regeneration
- Issue Date
- Acta Biomaterialia
- VOL 99-478
- In this study, we developed aptamer-conjugated hydroxyapatite (Apt-HA) that promotes bone regeneration and angiogenesis. The 3R02 bivalent aptamer specific to vascular endothelial growth factor (VEGF) was grafted to the hydroxyapatite (HA) surface. Apt-HA was tested for its VEGF protein capture ability to determine the optimal aptamer concentration immobilized on the HA. Apt-HA showed higher VEGF protein capture ability, and faster growth of human umbilical vein endothelial cell (HUVEC) compared to a neat HA with no cytotoxic effects on human osteoblasts. To examine in vivo angiogenesis and bone regeneration, Apt-HA and HA were bilaterally implanted into rabbit tibial metaphyseal defects and analyzed after eight weeks using micro-CT, histology, and histomorphometry. Apt-HA showed significantly increased the volume of new bones, the percentage of bone, and the density of bone mineral in cortical bone. Apt-HA also exhibited the enhanced bone formation at the cortical region in a histomorphometric analysis. Finally, Apt-HA showed significantly increased blood vessel number compared to a neat HA. In summary, the engineered Apt-HA has potential as a bone graft material that may simultaneously promote bone regeneration and angiogenesis.
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