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dc.contributor.author김병찬-
dc.contributor.author김혜리-
dc.contributor.author송민영-
dc.date.accessioned2021-06-09T04:23:58Z-
dc.date.available2021-06-09T04:23:58Z-
dc.date.issued2020-02-
dc.identifier.citationVOL 591, 113542-
dc.identifier.issn0003-2697-
dc.identifier.other54398-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/70952-
dc.description.abstractUsually, isolation of bacteria-speci&#64257-
dc.description.abstractc aptamers by SELEX is a time-consuming process due to the required repeated rounds of binding, separation, and ampli&#64257-
dc.description.abstractcation of the probes to target bacteria. Here, we show that it is possible to isolate bacteria-speci&#64257-
dc.description.abstractc DNA aptamers omitting the repeated rounds of binding incubation, separation, and ampli&#64257-
dc.description.abstractcation that are indispensable for SELEX. The serial removal of unbound DNAs to the bacterial cells from an initial mixture of bacteria and DNA libraries through serial centrifugation, one-time separation, and further one-time ampli&#64257-
dc.description.abstractcation of DNA bound to the target bacterial cells applied in this nonSELEX-based method allows successful aptamer isolation.-
dc.publisherAnalytical biochemistry-
dc.subjectCentrifugation-based partitioning-
dc.subjectRapid isolation-
dc.subjectBacteria-speci?c aptamer-
dc.subjectNon-SELEX-Based method-
dc.subjectEscherichia coli-
dc.titleRapid isolation of bacteria-specific aptamers with a non-SELEX-based method-
dc.typeArticle-
dc.relation.page113542-
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