Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method
- Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method
- 김병찬; 김혜리; 송민영
- Centrifugation-based partitioning; Rapid isolation; Bacteria-speci？c aptamer; Non-SELEX-Based method; Escherichia coli
- Issue Date
- Analytical biochemistry
- VOL 591, 113542
- Usually, isolation of bacteria-speciﬁ c aptamers by SELEX is a time-consuming process due to the required repeated rounds of binding, separation, and ampliﬁ cation of the probes to target bacteria. Here, we show that it is possible to isolate bacteria-speciﬁ c DNA aptamers omitting the repeated rounds of binding incubation, separation, and ampliﬁ cation that are indispensable for SELEX. The serial removal of unbound DNAs to the bacterial cells from an initial mixture of bacteria and DNA libraries through serial centrifugation, one-time separation, and further one-time ampliﬁ cation of DNA bound to the target bacterial cells applied in this nonSELEX-based method allows successful aptamer isolation.
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