Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method

Abstract

Usually, isolation of bacteria-speci&#64257

c aptamers by SELEX is a time-consuming process due to the required repeated rounds of binding, separation, and ampli&#64257

cation of the probes to target bacteria. Here, we show that it is possible to isolate bacteria-speci&#64257

c DNA aptamers omitting the repeated rounds of binding incubation, separation, and ampli&#64257

cation that are indispensable for SELEX. The serial removal of unbound DNAs to the bacterial cells from an initial mixture of bacteria and DNA libraries through serial centrifugation, one-time separation, and further one-time ampli&#64257

cation of DNA bound to the target bacterial cells applied in this nonSELEX-based method allows successful aptamer isolation.