Optimization, validation, and comparison of a rapid method for the quantification of insulinlike growth factor 1 in serum using liquid chromatography-high-resolution mass spectrometry
- Optimization, validation, and comparison of a rapid method for the quantification of insulinlike growth factor 1 in serum using liquid chromatography-high-resolution mass spectrometry
- 권오승; 민호필; 성창민; 김민영; 박지수; 서윤담; 이화정
- Doping; IGF-1; growth hormone; hGH biomarker
- Issue Date
- DRUG TESTING AND ANALYSIS
- VOL 13, NO 2-459
- Human insulinlike growth factor 1 (IGFI) is the primary mediator of the effects of the growth hormone (GH). Therefore, it has been used as a biomarker to detect the abuse of GH in sports. The measurement of IGFI relies on massbased and immunological approaches to analysis. Among the massbased analysis methods, liquid chromatography？mass spectrometry (LC？MS) has a number of functional advantages. LC？MS measurements based on the quantification of IGFI, according to trypsin digestion, are used in the most common method of analyzing doping. However, this method is timeconsuming and subject to experimental variability.In this study, we optimized a rapid method for detecting IGFI without the trypsin digestion step. This method of analysis uses an ultracentrifugal filter and an LCHRMS through narrowrange mass scan method. To verify the validity of this method, eight categories of validation testing were applied with the following results: linearity, R2 > 0.99; limit of detection,
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