Metal Enhanced Fluorescence of Rhodamine B Isothiocyanate from Micrometer-Sized Silver Powders
- Metal Enhanced Fluorescence of Rhodamine B Isothiocyanate from Micrometer-Sized Silver Powders
- 이지원; Kwan Kim; Yoon Mi Lee; Kuan Soo Shin
- Issue Date
- VOL 25, NO 5-2645
- We report here the use of micrometer-sized silver (mu Ag) powders to enhance the fluorescence emission of rhodamine B isothiocyanate (RhBITC). Our findings clearly show that the RhBITC displays up to a 36-fold increase in fluorescence emission intensity when RhBITC-labeled poly(allylamine hydrochloride) (RhBITC-PAH) is assembled onto a polyelectrolyte layer (about 30 nM thick) above mu Ag, when compared to that measured for RhBITC-PAH assembled directly onto a glass slide. A similar experiment conducted using a vacuum-evaporated Ag film displayed only a 5-fold increase. The enhanced fluorescence observed for mu Ag powders is obviously due to their ability to concentrate the electric fields. This suggests that metal-enhanced fluorescence (MEF) can potentially be employed to increase the sensitivity and detection limit of various bioassays that employ RhBITC as a fluorescence label. Since mu Ag powders are also an efficient Substrate for surface-enhanced Raman scattering (SERS), molecular sensors operating via both SERS and MEF may then be able to be fabricated using mu Ag powders.
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