Hydrangenol isolated from the leaves of Hydrangea serrata attenuates wrinkle formation and repairs skin moisture in UVB-irradiated hairless mi
- Hydrangenol isolated from the leaves of Hydrangea serrata attenuates wrinkle formation and repairs skin moisture in UVB-irradiated hairless mi
- 김형자; Da-Bin Myung; Hee-Soo Han; Ji-Sun Shin; Ji Yeon Park; Han Jun Hwang; Hye Shin Ahn; Sun Hee Lee; Kyung-Tae Lee
- Hydrangenol; Skin moisture; Ultraviolet B; Wrinkle formation; Photoaging; MAPK; MMPs; AP-1; Collagen
- Issue Date
- VOL 11-17
- Abstract: Our previous study showed that hydrangenol isolated from Hydrangea serrata leaves
exerts antiphotoaging activity in vitro. In this study, we determined its antiphotoaging effect in
UVB-irradiated HR-1 hairless mice. We evaluated wrinkle formation, skin thickness, histological
characteristics, and mRNA and protein expression using qRT-PCR and Western blot analysis in
dorsal skins. Hydrangenol mitigated wrinkle formation, dorsal thickness, dehydration, and collagen
degradation. Hydrangenol increased the expression of involucrin, filaggrin, and aquaporin-3
(AQP3) as well as hyaluronic acid (HA) production via hyaluronidase (HYAL)-1/-2 downregulation.
Consistent with the recovery of collagen composition, the expression of Pro-COL1A1 was increased
by hydrangenol. Matrix metalloproteinase (MMP)-1/-3, cyclooxygenase-2 (COX-2), and interleukin-6
(IL-6) expression was reduced by hydrangenol. Hydrangenol attenuated the phosphorylation of
mitogen-activated protein kinases (MAPKs) including ERK and p38, activator protein 1 (AP-1) subunit,
and signal transduction and activation of transcription 1 (STAT1). Hydrangenol upregulated the
expression of nuclear factor-E2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), NAD(P)H quinone
dehydrogenase 1 (NQO-1), glutamate cysteine ligase modifier subunit (GCLM), and glutamate
cysteine ligase catalysis subunit (GCLC). Taken together, our data suggest that hydrangenol can
prevent wrinkle formation by reducing MMP and inflammatory cytokine levels and increasing the
expression of moisturizing factors and antioxidant genes.
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