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dc.contributor.authorRahaman, Khandoker Asiqur-
dc.contributor.authorMuresan, Anca Raluca-
dc.contributor.authorMin, Ho phil-
dc.contributor.authorSON, Jung hyun-
dc.contributor.authorKang, Min-Jung-
dc.contributor.authorKwon, Oh Seung-
dc.date.accessioned2024-01-12T02:46:22Z-
dc.date.available2024-01-12T02:46:22Z-
dc.date.created2023-07-10-
dc.date.issued2023-05-25-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/76447-
dc.description.abstractThymosin β4 (Tβ4) was reported to exert beneficial bioactivities such as tissue repair, anti-inflammation, and reduced scar formation. It is listed as a prohibited substance in sports by the World Anti-Doping Agency. However, no metabolism studies of Tβ4 were reported yet. Previously, our lab reported in an in-vitro experiment that a total of 13 metabolites were found by using multiple enzymes, and six metabolites (Ac-Tβ31-43, Ac-Tβ1-11, Ac-Tβ17-43, Ac-Tβ1-14, Ac-Tβ1-15, and Ac-Tβ1-17) were confirmed by comparing with the synthetic standards. This study aimed to validate the metabolite analysis in rats urine and to develop a method for quantifying the metabolites. Among the six metabolites mentioned above, Ac-Tβ1-14 was a common metabolite in the pure enzyme and microsome systems, huvec cells, and rat urine samples of rats intraperitoneally treated with Ac-Tβ4. A method for detecting and quantifying Ac-Tβ1-14 was developed and validated using Q-Exactive orbitrap mass spectrometry. LOD and LOQ of the Ac-Tβ1-14 were determined to be 0.19 and 0.58 ng/ml and showed good linearity (r2 = 0.9998). Among 3 in-vitro systems, LAP produces the highest amount of Ac-Tβ1-14, compared to HKM and huvec cells. The metabolite Ac-Tβ1-14 was quantitatively determined by 48 hr in rats, with the highest concentration occurring between 0-6 hr. These results suggest that the metabolite should be a good tool for detecting Ac-Tβ1-14 in urine, a potential biomarker for screening the parent Tβ4 in doping tests.-
dc.languageKorean-
dc.publisher한국분석과학회-
dc.titleQuantitative determination of the metabolite Ac-Tβ1-14 in in-vitro and urine of rats treated with Thymosin β4 by LC Orbitrap HR-MS/MS-
dc.typeConference-
dc.description.journalClass2-
dc.identifier.bibliographicCitation제70회 한국분석과학회 춘계 학술대회-
dc.citation.title제70회 한국분석과학회 춘계 학술대회-
dc.citation.conferencePlaceKO-
dc.citation.conferencePlace강릉 세인트존스호텔-
dc.citation.conferenceDate2023-05-24-
dc.relation.isPartOf제70회 한국분석과학회 춘계 학술대회-

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