Microfluidic platforms with monolithically integrated hierarchical apertures for the facile and rapid formation of cargo-carrying vesicles

Abstract

We fabricated a simple yet robust microfluidic platform with monolithically integrated hierarchical apertures. This platform showed efficient diffusive mixing of the introduced lipids through approximately 8000 divisions with tiny pores (similar to 5 mu m in diameter), resulting in massive, real-time production of various cargo-carrying particles via multi-hydrodynamic focusing.