Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Choi, Jung-Kyun | - |
dc.contributor.author | Chung, Haeun | - |
dc.contributor.author | Oh, Seung Ja | - |
dc.contributor.author | Kim, Jong-Wan | - |
dc.contributor.author | Kim, Sang-Heon | - |
dc.date.accessioned | 2024-01-19T09:02:37Z | - |
dc.date.available | 2024-01-19T09:02:37Z | - |
dc.date.created | 2023-08-02 | - |
dc.date.issued | 2023-08 | - |
dc.identifier.issn | 1742-7061 | - |
dc.identifier.uri | https://pubs.kist.re.kr/handle/201004/113433 | - |
dc.description.abstract | Stem cell therapy has emerged as a promising regenerative medicine strategy but is limited by poor cell survival, leading to low therapeutic outcomes. We developed cell spheroid therapeutics to overcome this limitation. We utilized solid-phase FGF2 to form functionally enhanced cell spheroid-adipose derived (FECS-Ad), a type of cell spheroid that preconditions cells with intrinsic hypoxia to increase the survival of transplanted cells. We demonstrated an increase in hypoxia-inducible factor 1-alpha (HIF-1 & alpha;) levels in FECS-Ad, which led to the upregulation of tissue inhibitor of metalloproteinase 1 (TIMP1). TIMP1 enhanced the survival of FECS-Ad, presumably through the CD63/FAK/Akt/Bcl2 anti-apoptotic signaling pathway. Cell viability of transplanted FECS-Ad was reduced by TIMP1 knockdown in an in vitro collagen gel block and a mouse model of critical limb ischemia (CLI). TIMP1 knockdown in FECS-Ad inhibited angiogenesis and muscle regeneration induced by FECS-Ad transplanted into ischemic mouse tissue. Genetic overexpression of TIMP1 in FECS-Ad further promoted the survival and therapeutic efficacy of transplanted FECS-Ad. Collectively, we suggest that TIMP1 acts as a key survival factor to improve the survival of transplanted stem cell spheroids, which provides scientific evidence for enhanced therapeutic efficacy of stem cell spheroids, and FECS-Ad as a potential therapeutic agent to treat CLI. | - |
dc.language | English | - |
dc.publisher | Elsevier BV | - |
dc.title | Functionally enhanced cell spheroids for stem cell therapy: Role of TIMP1 in the survival and therapeutic effectiveness of stem cell spheroids | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.actbio.2023.05.033 | - |
dc.description.journalClass | 1 | - |
dc.identifier.bibliographicCitation | Acta Biomaterialia, v.166, pp.454 - 469 | - |
dc.citation.title | Acta Biomaterialia | - |
dc.citation.volume | 166 | - |
dc.citation.startPage | 454 | - |
dc.citation.endPage | 469 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.identifier.wosid | 001031572300001 | - |
dc.identifier.scopusid | 2-s2.0-85163348050 | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | BREAST EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | TISSUE INHIBITOR | - |
dc.subject.keywordPlus | INFARCTED HEART | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | ADHESION | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | METALLOPROTEINASE-1 | - |
dc.subject.keywordAuthor | Tissue inhibitor matrix metalloproteinase 1 | - |
dc.subject.keywordAuthor | Spheroid | - |
dc.subject.keywordAuthor | Critical limb ischemia | - |
dc.subject.keywordAuthor | Stem cell therapy | - |
dc.subject.keywordAuthor | Survival | - |
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