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dc.contributor.authorKim, Hyunbin-
dc.contributor.authorNam, Min-Ho-
dc.contributor.authorJeong, Sohyeon-
dc.contributor.authorLee, Hyowon-
dc.contributor.authorOh, Soo-Jin-
dc.contributor.authorKim, Jeongjin-
dc.contributor.authorChoi, Nakwon-
dc.contributor.authorSeong, Jihye-
dc.date.accessioned2024-01-19T12:01:08Z-
dc.date.available2024-01-19T12:01:08Z-
dc.date.created2022-06-23-
dc.date.issued2022-06-
dc.identifier.issn0301-0082-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/115144-
dc.description.abstractDopaminergic signaling is regulated by transient micromolar (phasic) and background nanomolar (tonic) dopamine releases in the brain. These dopamine signals can be differentially translated by dopamine receptor type 1 and type 2, DRD1 and DRD2, which are G protein-coupled receptors (GPCRs). In response to dopamine, DRD1 and DRD2 are known to mediate opposite functions on cAMP production via Gs and Gi protein signaling. Interestingly, they can form a heterodimer. However, receptor crosstalk between DRD1-DRD2 heterodimers has not been directly measured, but it was only inferred from measuring downstream signaling pathways. Here we develop fluorescent protein-based multicolor biosensors which can monitor individual activation states of DRD1 and DRD2, and apply them to directly monitor the functional crosstalk between DRD1-DRD2 heterodimers in live cells. Utilizing these powerful tools, we surprisingly discover differential crosstalk in the DRD1-DRD2 heterodimers upon different dopamine (DA) levels: DRD1 activation is selectively inhibited at micromolar DA levels, while DRD2 is inhibited only by nanomolar DA concentration, implying a novel function of the DRD1-DRD2 heterodimer upon different DA levels. Our results imply differential receptor crosstalk and novel functions of the DRD1-DRD2 heterodimer in response to physiological dopamine levels from nanomolar to micromolar dopamine concentrations.-
dc.languageEnglish-
dc.publisherPergamon Press Ltd.-
dc.titleVisualization of differential GPCR crosstalk in DRD1-DRD2 heterodimer upon different dopamine levels-
dc.typeArticle-
dc.identifier.doi10.1016/j.pneurobio.2022.102266-
dc.description.journalClass1-
dc.identifier.bibliographicCitationProgress in Neurobiology, v.213-
dc.citation.titleProgress in Neurobiology-
dc.citation.volume213-
dc.description.isOpenAccessY-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.identifier.wosid000805977700003-
dc.relation.journalWebOfScienceCategoryNeurosciences-
dc.relation.journalResearchAreaNeurosciences & Neurology-
dc.type.docTypeReview-
dc.subject.keywordPlusD1-D2 RECEPTOR HETEROMER-
dc.subject.keywordPlusAFFINITY-
dc.subject.keywordPlusPHARMACOLOGY-
dc.subject.keywordPlusACTIVATION-
dc.subject.keywordPlusNEURONS-
dc.subject.keywordPlusD-1-
dc.subject.keywordPlusD1-
dc.subject.keywordPlusHETEROOLIGOMERS-
dc.subject.keywordPlusRELEASE-
dc.subject.keywordPlusSYSTEM-
dc.subject.keywordAuthorGPCR heterodimer-
dc.subject.keywordAuthorDopamine receptor sensor-
dc.subject.keywordAuthorDRD1-
dc.subject.keywordAuthorDRD2-
dc.subject.keywordAuthorcAMP-
dc.subject.keywordAuthorTonic and phasic DA release-
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