Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yang, Hee Seok | - |
dc.contributor.author | Park, Kwideok | - |
dc.contributor.author | Kim, Byoung-Soo | - |
dc.contributor.author | Kim, Jae-Jin | - |
dc.contributor.author | Ahn, Kwang-Duk | - |
dc.contributor.author | Han, Dong Keun | - |
dc.date.accessioned | 2024-01-19T13:39:02Z | - |
dc.date.available | 2024-01-19T13:39:02Z | - |
dc.date.created | 2022-03-07 | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1013-9826 | - |
dc.identifier.uri | https://pubs.kist.re.kr/handle/201004/116437 | - |
dc.description.abstract | Nonporous PLLA film and porous PLLA scaffolds were prepared and then grafted with acrylic acid (AA) using in situ direct plasma treatment to obtain PLLA-g-PAA. Chondrocytes isolated from rabbit knee articular cartilages were cultivated in Dulbecco's modified eagle medium-F12 (DMEM-F12) containing 10% fetal bovine serum (FBS) and 1% antibiotics and passaged twice before cell seeding. Once seeded on either PLLA films or scaffolds, they were placed in a bioreactor system and an intermittent hydrodynamic pressure (IHP) was applied in 3 bars, while turned on for 2 min and off for 28 min during 15-day culture. AA grafting to PLLA surface was confirmed from various surface analyses. From WST-1 assay, chondrocyte proliferation was significantly improved with dynamic IHP for PLLA and PLLA-g-PAA scaffolds as compared to static culture. This study indicates that IHP may have significant influence on chondrocytes behavior in 3D culture environment. | - |
dc.language | English | - |
dc.publisher | TRANS TECH PUBLICATIONS LTD | - |
dc.title | In vitro chondrocyte culture using hydrophilized PLLA scaffold in bioreactor system | - |
dc.type | Conference | - |
dc.identifier.doi | 10.4028/www.scientific.net/KEM.342-343.253 | - |
dc.description.journalClass | 1 | - |
dc.identifier.bibliographicCitation | 7th Asian Symposium on Biomedical Materials (ASBM7), v.342-343, pp.253 - + | - |
dc.citation.title | 7th Asian Symposium on Biomedical Materials (ASBM7) | - |
dc.citation.volume | 342-343 | - |
dc.citation.startPage | 253 | - |
dc.citation.endPage | + | - |
dc.citation.conferencePlace | SZ | - |
dc.citation.conferencePlace | Cheju Isl, SOUTH KOREA | - |
dc.citation.conferenceDate | 2006-08-20 | - |
dc.relation.isPartOf | ASBM7: ADVANCED BIOMATERIALS VII | - |
dc.identifier.wosid | 000246657900064 | - |
dc.identifier.scopusid | 2-s2.0-34147166241 | - |
dc.type.docType | Proceedings Paper | - |
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