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dc.contributor.authorYang, Hee Seok-
dc.contributor.authorPark, Kwideok-
dc.contributor.authorKim, Byoung-Soo-
dc.contributor.authorKim, Jae-Jin-
dc.contributor.authorAhn, Kwang-Duk-
dc.contributor.authorHan, Dong Keun-
dc.date.accessioned2024-01-19T13:39:02Z-
dc.date.available2024-01-19T13:39:02Z-
dc.date.created2022-03-07-
dc.date.issued2007-
dc.identifier.issn1013-9826-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/116437-
dc.description.abstractNonporous PLLA film and porous PLLA scaffolds were prepared and then grafted with acrylic acid (AA) using in situ direct plasma treatment to obtain PLLA-g-PAA. Chondrocytes isolated from rabbit knee articular cartilages were cultivated in Dulbecco's modified eagle medium-F12 (DMEM-F12) containing 10% fetal bovine serum (FBS) and 1% antibiotics and passaged twice before cell seeding. Once seeded on either PLLA films or scaffolds, they were placed in a bioreactor system and an intermittent hydrodynamic pressure (IHP) was applied in 3 bars, while turned on for 2 min and off for 28 min during 15-day culture. AA grafting to PLLA surface was confirmed from various surface analyses. From WST-1 assay, chondrocyte proliferation was significantly improved with dynamic IHP for PLLA and PLLA-g-PAA scaffolds as compared to static culture. This study indicates that IHP may have significant influence on chondrocytes behavior in 3D culture environment.-
dc.languageEnglish-
dc.publisherTRANS TECH PUBLICATIONS LTD-
dc.titleIn vitro chondrocyte culture using hydrophilized PLLA scaffold in bioreactor system-
dc.typeConference-
dc.identifier.doi10.4028/www.scientific.net/KEM.342-343.253-
dc.description.journalClass1-
dc.identifier.bibliographicCitation7th Asian Symposium on Biomedical Materials (ASBM7), v.342-343, pp.253 - +-
dc.citation.title7th Asian Symposium on Biomedical Materials (ASBM7)-
dc.citation.volume342-343-
dc.citation.startPage253-
dc.citation.endPage+-
dc.citation.conferencePlaceSZ-
dc.citation.conferencePlaceCheju Isl, SOUTH KOREA-
dc.citation.conferenceDate2006-08-20-
dc.relation.isPartOfASBM7: ADVANCED BIOMATERIALS VII-
dc.identifier.wosid000246657900064-
dc.identifier.scopusid2-s2.0-34147166241-
dc.type.docTypeProceedings Paper-
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KIST Conference Paper > 2007
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