Full metadata record

DC Field Value Language
dc.contributor.authorKim, Woojun-
dc.contributor.authorYoon, Hong Yeol-
dc.contributor.authorLim, Seungho-
dc.contributor.authorStayton, Patrick S.-
dc.contributor.authorKim, In-San-
dc.contributor.authorKim, Kwangmeyung-
dc.contributor.authorKwon, Ick Chan-
dc.date.accessioned2024-01-19T15:33:19Z-
dc.date.available2024-01-19T15:33:19Z-
dc.date.created2021-09-02-
dc.date.issued2021-01-10-
dc.identifier.issn0168-3659-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/117540-
dc.description.abstractNon-invasive tracking of T-cells may help to predict the patient responsiveness and therapeutic outcome. Herein, we developed bioorthogonal T-cell labeling and tracking strategy using bioorthogonal click chemistry. First, ovalbumin (OVA) antigen-specific cytotoxic T-cells (CTLs) were incubated with N-azidoacetyl-D-mannosamine-tetraacylated (Ac(4)ManNAz) for incorporating azide (-N-3) groups on the surface of CTLs via metabolic glyco-engineering. Subsequently, azide groups on the CTLs were chemically labeled with near infrared fluorescence (NIRF) dye, Cy5.5, conjugated dibenzylcyclooctyne (DBCO-Cy5.5) via bioorthogonal click chemistry, resulting in Cy5.5-labeled CTLs (Cy5.5-CTLs). The labeling efficiency of Cy5.5-CTLs could be readily controlled by changing concentrations of Ac(4)ManNAz and DBCO-Cy5.5 in cultured cells. Importantly, Cy5.5-CTLs presented the strong NIRF signals in vitro and they showed no significant changes in the functional properties, such as cell viability, proliferation, and antigen-specific cytolytic activity. In ovalbumin (OVA)-expressing E.G-7 tumor-bearing immune-deficient mice, intravenously injected Cy5.5-CTLs were clearly observed at targeted solid tumors via non-invasive NIRF imaging. Moreover, tumor growth inhibition of E.G-7 tumors was closely correlated with the intensity of NIRF signals from Cy5.5-CTLs at tumors after 2-3 days post-injection. The Cy5.5-CTLs showed different therapeutic responses in E.G-7 tumor-bearing immune-competent mice, in which they were divided by their tumor growth efficacy as 'high therapeutic response (TR (+))' and 'low therapeutic response (TR (-))'. These different therapeutic responses of Cy5.5-CTLs were highly correlated with the NIRF signals of Cy5.5-CTLs at targeted tumor tissues in the early stage. Therefore, non-invasive tracking of T-cells can be able to predict and elicit therapeutic responses in the adoptive T-cell therapy.-
dc.languageEnglish-
dc.publisherELSEVIER-
dc.titleIn vivo tracking of bioorthogonally labeled T-cells for predicting therapeutic efficacy of adoptive T-cell therapy-
dc.typeArticle-
dc.identifier.doi10.1016/j.jconrel.2020.12.002-
dc.description.journalClass1-
dc.identifier.bibliographicCitationJOURNAL OF CONTROLLED RELEASE, v.329, pp.223 - 236-
dc.citation.titleJOURNAL OF CONTROLLED RELEASE-
dc.citation.volume329-
dc.citation.startPage223-
dc.citation.endPage236-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.identifier.wosid000626334700016-
dc.identifier.scopusid2-s2.0-85097341070-
dc.relation.journalWebOfScienceCategoryChemistry, Multidisciplinary-
dc.relation.journalWebOfScienceCategoryPharmacology & Pharmacy-
dc.relation.journalResearchAreaChemistry-
dc.relation.journalResearchAreaPharmacology & Pharmacy-
dc.type.docTypeArticle-
dc.subject.keywordAuthorAdoptive T-cell therapy-
dc.subject.keywordAuthorBioorthogonal click chemistry-
dc.subject.keywordAuthorT-cell tracking-
dc.subject.keywordAuthorin vivo imaging-
Appears in Collections:
KIST Article > 2021
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE