Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Taejin | - |
dc.contributor.author | Park, Jin-Soo | - |
dc.contributor.author | Sim, Ji Han | - |
dc.contributor.author | Kim, Seung-Young | - |
dc.date.accessioned | 2024-01-19T17:30:28Z | - |
dc.date.available | 2024-01-19T17:30:28Z | - |
dc.date.created | 2021-08-31 | - |
dc.date.issued | 2020-07 | - |
dc.identifier.issn | 1420-3049 | - |
dc.identifier.uri | https://pubs.kist.re.kr/handle/201004/118506 | - |
dc.description.abstract | Acetylation involves the chemical introduction of an acetyl group in place of an active hydrogen group into a compound. In this study, we synthesized 7-acetoxycoumarin (7AC) from acetylation of umbelliferone (UMB). We examined the anti-inflammatory properties of 7AC in lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells. The anti-inflammatory activity of 7AC on viability of treated cells was assessed by measuring the level of expression of NO, PGE(2)and pro-inflammatory cytokines, namely interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) in 7AC-treated RAW 264.7 macrophages. The 7AC was nontoxic to cells and inhibited the production of cytokines in a concentration-dependent manner. In addition, its treatment suppressed the production of pro-inflammatory cytokines in a dose-dependent manner and concomitantly decreased the protein and mRNA expressions of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2). Moreover, the levels of the phosphorylation of mitogen-activated protein kinase (MAPK) family proteins such as extracellular signal-regulated kinase (ERK), c-JunN-terminal kinase (JNK), p38 and nuclear factor kappa B (NF-kappa B) were reduced by 7AC. In conclusion, we generated an anti-inflammatory compound through acetylation and demonstrated its efficacy in cell-based in vitro assays. | - |
dc.language | English | - |
dc.publisher | MDPI | - |
dc.subject | NITRIC-OXIDE | - |
dc.subject | TNF-ALPHA | - |
dc.subject | UMBELLIFERONE | - |
dc.subject | METABOLISM | - |
dc.subject | EXPRESSION | - |
dc.subject | INDUCTION | - |
dc.subject | MECHANISM | - |
dc.subject | INNATE | - |
dc.subject | KINASE | - |
dc.subject | INOS | - |
dc.title | 7-Acetoxycoumarin Inhibits LPS-Induced Inflammatory Cytokine Synthesis by I kappa B alpha Degradation and MAPK Activation in Macrophage Cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/molecules25143124 | - |
dc.description.journalClass | 1 | - |
dc.identifier.bibliographicCitation | MOLECULES, v.25, no.14 | - |
dc.citation.title | MOLECULES | - |
dc.citation.volume | 25 | - |
dc.citation.number | 14 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.identifier.wosid | 000554266400001 | - |
dc.identifier.scopusid | 2-s2.0-85088203367 | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | NITRIC-OXIDE | - |
dc.subject.keywordPlus | TNF-ALPHA | - |
dc.subject.keywordPlus | UMBELLIFERONE | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | INNATE | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | INOS | - |
dc.subject.keywordAuthor | 7-acetoxycoumarin | - |
dc.subject.keywordAuthor | umbelliferone | - |
dc.subject.keywordAuthor | anti-inflammatory | - |
dc.subject.keywordAuthor | acetylation | - |
dc.subject.keywordAuthor | phosphorylation | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.