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dc.contributor.authorPark, Taejin-
dc.contributor.authorPark, Jin-Soo-
dc.contributor.authorSim, Ji Han-
dc.contributor.authorKim, Seung-Young-
dc.date.accessioned2024-01-19T17:30:28Z-
dc.date.available2024-01-19T17:30:28Z-
dc.date.created2021-08-31-
dc.date.issued2020-07-
dc.identifier.issn1420-3049-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/118506-
dc.description.abstractAcetylation involves the chemical introduction of an acetyl group in place of an active hydrogen group into a compound. In this study, we synthesized 7-acetoxycoumarin (7AC) from acetylation of umbelliferone (UMB). We examined the anti-inflammatory properties of 7AC in lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells. The anti-inflammatory activity of 7AC on viability of treated cells was assessed by measuring the level of expression of NO, PGE(2)and pro-inflammatory cytokines, namely interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) in 7AC-treated RAW 264.7 macrophages. The 7AC was nontoxic to cells and inhibited the production of cytokines in a concentration-dependent manner. In addition, its treatment suppressed the production of pro-inflammatory cytokines in a dose-dependent manner and concomitantly decreased the protein and mRNA expressions of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2). Moreover, the levels of the phosphorylation of mitogen-activated protein kinase (MAPK) family proteins such as extracellular signal-regulated kinase (ERK), c-JunN-terminal kinase (JNK), p38 and nuclear factor kappa B (NF-kappa B) were reduced by 7AC. In conclusion, we generated an anti-inflammatory compound through acetylation and demonstrated its efficacy in cell-based in vitro assays.-
dc.languageEnglish-
dc.publisherMDPI-
dc.subjectNITRIC-OXIDE-
dc.subjectTNF-ALPHA-
dc.subjectUMBELLIFERONE-
dc.subjectMETABOLISM-
dc.subjectEXPRESSION-
dc.subjectINDUCTION-
dc.subjectMECHANISM-
dc.subjectINNATE-
dc.subjectKINASE-
dc.subjectINOS-
dc.title7-Acetoxycoumarin Inhibits LPS-Induced Inflammatory Cytokine Synthesis by I kappa B alpha Degradation and MAPK Activation in Macrophage Cells-
dc.typeArticle-
dc.identifier.doi10.3390/molecules25143124-
dc.description.journalClass1-
dc.identifier.bibliographicCitationMOLECULES, v.25, no.14-
dc.citation.titleMOLECULES-
dc.citation.volume25-
dc.citation.number14-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.identifier.wosid000554266400001-
dc.identifier.scopusid2-s2.0-85088203367-
dc.relation.journalWebOfScienceCategoryBiochemistry & Molecular Biology-
dc.relation.journalWebOfScienceCategoryChemistry, Multidisciplinary-
dc.relation.journalResearchAreaBiochemistry & Molecular Biology-
dc.relation.journalResearchAreaChemistry-
dc.type.docTypeArticle-
dc.subject.keywordPlusNITRIC-OXIDE-
dc.subject.keywordPlusTNF-ALPHA-
dc.subject.keywordPlusUMBELLIFERONE-
dc.subject.keywordPlusMETABOLISM-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusINDUCTION-
dc.subject.keywordPlusMECHANISM-
dc.subject.keywordPlusINNATE-
dc.subject.keywordPlusKINASE-
dc.subject.keywordPlusINOS-
dc.subject.keywordAuthor7-acetoxycoumarin-
dc.subject.keywordAuthorumbelliferone-
dc.subject.keywordAuthoranti-inflammatory-
dc.subject.keywordAuthoracetylation-
dc.subject.keywordAuthorphosphorylation-
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