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dc.contributor.authorPark, Min Gu-
dc.contributor.authorJang, Heeyeong-
dc.contributor.authorLee, Sang-Hoon-
dc.contributor.authorLee, C. Justin-
dc.date.accessioned2024-01-20T02:00:59Z-
dc.date.available2024-01-20T02:00:59Z-
dc.date.created2021-09-01-
dc.date.issued2017-04-
dc.identifier.issn1226-2560-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/122923-
dc.description.abstractRadial glial cells (RGCs) which function as neural stem cells are known to be non-excitable and their proliferation depends on the intracellular calcium (Ca2+) level. It has been well established that Inositol 1,4,5-trisphosphate (IP3)-mediated Ca2+ release and Ca2+ entry through various Ca2+ channels are involved in the proliferation of RGCs. Furthermore, RGCs line the ventricular wall and are exposed to a shear stress due to a physical contact with the cerebrospinal fluid (CSF). However, little is known about how the Ca2+ entry through mechanosensitive ion channels affects the proliferation of RGCs. Hence, we hypothesized that shear stress due to a flow of CSF boosts the proliferative potential of RGCs possibly via an activation of mechanosensitive Ca2+ channel during the embryonic brain development. Here, we developed a new microfluidic two-dimensional culture system to establish a link between the flow shear stress and the proliferative activity of cultured RGCs. Using this microfluidic device, we successfully visualized the artificial CSF and RGCs in direct contact and found a significant enhancement of proliferative capacity of RGCs in response to increased shear stress. To determine if there are any mechanosensitive ion channels involved, a mechanical stimulation by poking was given to individual RGCs. We found that a poking on radial glial cell induced an increase in intracellular Ca2+ level, which disappeared under the extracellular Ca2+-free condition. Our results suggest that the shear stress by CSF flow possibly activates mechanosensitive Ca2+ channels, which gives rise to a Ca2+ entry which enhances the proliferative capacity of RGCs.-
dc.languageEnglish-
dc.publisherKOREAN SOC BRAIN & NEURAL SCIENCE, KOREAN SOC NEURODEGENERATIVE DISEASE-
dc.subjectNEURAL STEM-CELLS-
dc.subjectPRIMARY CILIA-
dc.subjectBRAIN GROWTH-
dc.subjectEXPRESSION-
dc.subjectCYCLE-
dc.subjectPROGENITORS-
dc.subjectEPITHELIUM-
dc.subjectINCREASES-
dc.subjectEMBRYO-
dc.titleFlow Shear Stress Enhances the Proliferative Potential of Cultured Radial Glial Cells Possibly Via an Activation of Mechanosensitive Calcium Channel-
dc.typeArticle-
dc.identifier.doi10.5607/en.2017.26.2.71-
dc.description.journalClass1-
dc.identifier.bibliographicCitationEXPERIMENTAL NEUROBIOLOGY, v.26, no.2, pp.71 - 81-
dc.citation.titleEXPERIMENTAL NEUROBIOLOGY-
dc.citation.volume26-
dc.citation.number2-
dc.citation.startPage71-
dc.citation.endPage81-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.description.journalRegisteredClasskci-
dc.identifier.kciidART002221537-
dc.identifier.wosid000406862500001-
dc.identifier.scopusid2-s2.0-85018679357-
dc.relation.journalWebOfScienceCategoryMedicine, Research & Experimental-
dc.relation.journalWebOfScienceCategoryNeurosciences-
dc.relation.journalResearchAreaResearch & Experimental Medicine-
dc.relation.journalResearchAreaNeurosciences & Neurology-
dc.type.docTypeArticle-
dc.subject.keywordPlusNEURAL STEM-CELLS-
dc.subject.keywordPlusPRIMARY CILIA-
dc.subject.keywordPlusBRAIN GROWTH-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusCYCLE-
dc.subject.keywordPlusPROGENITORS-
dc.subject.keywordPlusEPITHELIUM-
dc.subject.keywordPlusINCREASES-
dc.subject.keywordPlusEMBRYO-
dc.subject.keywordAuthorRadial glial cell-
dc.subject.keywordAuthorShear stress-
dc.subject.keywordAuthorMechanosensitive ion channel-
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KIST Article > 2017
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