Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Park, Min Gu | - |
dc.contributor.author | Jang, Heeyeong | - |
dc.contributor.author | Lee, Sang-Hoon | - |
dc.contributor.author | Lee, C. Justin | - |
dc.date.accessioned | 2024-01-20T02:00:59Z | - |
dc.date.available | 2024-01-20T02:00:59Z | - |
dc.date.created | 2021-09-01 | - |
dc.date.issued | 2017-04 | - |
dc.identifier.issn | 1226-2560 | - |
dc.identifier.uri | https://pubs.kist.re.kr/handle/201004/122923 | - |
dc.description.abstract | Radial glial cells (RGCs) which function as neural stem cells are known to be non-excitable and their proliferation depends on the intracellular calcium (Ca2+) level. It has been well established that Inositol 1,4,5-trisphosphate (IP3)-mediated Ca2+ release and Ca2+ entry through various Ca2+ channels are involved in the proliferation of RGCs. Furthermore, RGCs line the ventricular wall and are exposed to a shear stress due to a physical contact with the cerebrospinal fluid (CSF). However, little is known about how the Ca2+ entry through mechanosensitive ion channels affects the proliferation of RGCs. Hence, we hypothesized that shear stress due to a flow of CSF boosts the proliferative potential of RGCs possibly via an activation of mechanosensitive Ca2+ channel during the embryonic brain development. Here, we developed a new microfluidic two-dimensional culture system to establish a link between the flow shear stress and the proliferative activity of cultured RGCs. Using this microfluidic device, we successfully visualized the artificial CSF and RGCs in direct contact and found a significant enhancement of proliferative capacity of RGCs in response to increased shear stress. To determine if there are any mechanosensitive ion channels involved, a mechanical stimulation by poking was given to individual RGCs. We found that a poking on radial glial cell induced an increase in intracellular Ca2+ level, which disappeared under the extracellular Ca2+-free condition. Our results suggest that the shear stress by CSF flow possibly activates mechanosensitive Ca2+ channels, which gives rise to a Ca2+ entry which enhances the proliferative capacity of RGCs. | - |
dc.language | English | - |
dc.publisher | KOREAN SOC BRAIN & NEURAL SCIENCE, KOREAN SOC NEURODEGENERATIVE DISEASE | - |
dc.subject | NEURAL STEM-CELLS | - |
dc.subject | PRIMARY CILIA | - |
dc.subject | BRAIN GROWTH | - |
dc.subject | EXPRESSION | - |
dc.subject | CYCLE | - |
dc.subject | PROGENITORS | - |
dc.subject | EPITHELIUM | - |
dc.subject | INCREASES | - |
dc.subject | EMBRYO | - |
dc.title | Flow Shear Stress Enhances the Proliferative Potential of Cultured Radial Glial Cells Possibly Via an Activation of Mechanosensitive Calcium Channel | - |
dc.type | Article | - |
dc.identifier.doi | 10.5607/en.2017.26.2.71 | - |
dc.description.journalClass | 1 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL NEUROBIOLOGY, v.26, no.2, pp.71 - 81 | - |
dc.citation.title | EXPERIMENTAL NEUROBIOLOGY | - |
dc.citation.volume | 26 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 71 | - |
dc.citation.endPage | 81 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.identifier.kciid | ART002221537 | - |
dc.identifier.wosid | 000406862500001 | - |
dc.identifier.scopusid | 2-s2.0-85018679357 | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.relation.journalWebOfScienceCategory | Neurosciences | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | NEURAL STEM-CELLS | - |
dc.subject.keywordPlus | PRIMARY CILIA | - |
dc.subject.keywordPlus | BRAIN GROWTH | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CYCLE | - |
dc.subject.keywordPlus | PROGENITORS | - |
dc.subject.keywordPlus | EPITHELIUM | - |
dc.subject.keywordPlus | INCREASES | - |
dc.subject.keywordPlus | EMBRYO | - |
dc.subject.keywordAuthor | Radial glial cell | - |
dc.subject.keywordAuthor | Shear stress | - |
dc.subject.keywordAuthor | Mechanosensitive ion channel | - |
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