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dc.contributor.authorKim, Min Ju-
dc.contributor.authorPark, Jong-Sung-
dc.contributor.authorLee, So Jin-
dc.contributor.authorJang, Jiyeon-
dc.contributor.authorPark, Jin Su-
dc.contributor.authorBack, Seung Hyun-
dc.contributor.authorBahn, Gahee-
dc.contributor.authorPark, Jae Hyung-
dc.contributor.authorKang, Young Mo-
dc.contributor.authorKim, Sun Hwa-
dc.contributor.authorKwon, Ick Chan-
dc.contributor.authorJo, Dong-Gyu-
dc.contributor.authorKim, Kwangmeyung-
dc.date.accessioned2024-01-20T06:00:59Z-
dc.date.available2024-01-20T06:00:59Z-
dc.date.created2021-09-04-
dc.date.issued2015-10-28-
dc.identifier.issn0168-3659-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/124867-
dc.description.abstractNotch pathway plays a pivotal role in synoviocytes involved in progression of rheumatoid arthritis (RA). Herein, we designed the Notch1 targeting siRNA delivery nanoparticles (siRNA-NPs) in order to confirm the anti-inflammatory effect in collagen-induced arthritis (CIA) model. The siRNA-NPs were successfully produced by encapsulating polymerized siRNA (poly-siRNA) into thiolated glycol chitosan (tGC) nanoparticles in aqueous condition. The in vitro Notch1 inhibition of siRNA-NPs in murine macrophage cell (RAW264.7) was confirmed using confocal microscopy and real time PCR. Fluorescently labeled siRNA-NPs were successfully transfected in RAW264.7 and modulated the expression of Notch1 in mRNA level. For in vivo study, siRNA-NPs exhibited the higher targeting efficiency in the arthritic joins of CIA mice, confirmed by the near-infrared fluorescence (NIRF) imaging. Furthermore, inhibition of Notch1 with siRNA-NPs resulted in retarded progression of inflammation, bone erosion, and cartilage damage in CIA mice. Novel Notch1 targeting siRNA delivery system of siRNA-NPs showed effective RA treatment by suppressing Notch1 signaling pathway without undesirable severe toxicity. Thus, Notch1 inhibiting siRNA-NPs demonstrated the great potential in RA therapeutics that was hard to be achieved using conventional drugs. (C) 2015 Elsevier B.V. All rights reserved.-
dc.languageEnglish-
dc.publisherELSEVIER SCIENCE BV-
dc.subjectCOLLAGEN-INDUCED ARTHRITIS-
dc.subjectGLYCOL CHITOSAN NANOPARTICLES-
dc.subjectPOLYMERIZED SIRNA-
dc.subjectINTRACELLULAR DOMAIN-
dc.subjectSYSTEMIC DELIVERY-
dc.subjectCELLULAR UPTAKE-
dc.subjectANGIOGENESIS-
dc.subjectPATHOGENESIS-
dc.subjectINFLAMMATION-
dc.subjectACTIVATION-
dc.titleNotch1 targeting siRNA delivery nanoparticles for rheumatoid arthritis therapy-
dc.typeArticle-
dc.identifier.doi10.1016/j.jconrel.2015.08.025-
dc.description.journalClass1-
dc.identifier.bibliographicCitationJOURNAL OF CONTROLLED RELEASE, v.216, pp.140 - 148-
dc.citation.titleJOURNAL OF CONTROLLED RELEASE-
dc.citation.volume216-
dc.citation.startPage140-
dc.citation.endPage148-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.identifier.wosid000363480600015-
dc.identifier.scopusid2-s2.0-84940199341-
dc.relation.journalWebOfScienceCategoryChemistry, Multidisciplinary-
dc.relation.journalWebOfScienceCategoryPharmacology & Pharmacy-
dc.relation.journalResearchAreaChemistry-
dc.relation.journalResearchAreaPharmacology & Pharmacy-
dc.type.docTypeArticle-
dc.subject.keywordPlusCOLLAGEN-INDUCED ARTHRITIS-
dc.subject.keywordPlusGLYCOL CHITOSAN NANOPARTICLES-
dc.subject.keywordPlusPOLYMERIZED SIRNA-
dc.subject.keywordPlusINTRACELLULAR DOMAIN-
dc.subject.keywordPlusSYSTEMIC DELIVERY-
dc.subject.keywordPlusCELLULAR UPTAKE-
dc.subject.keywordPlusANGIOGENESIS-
dc.subject.keywordPlusPATHOGENESIS-
dc.subject.keywordPlusINFLAMMATION-
dc.subject.keywordPlusACTIVATION-
dc.subject.keywordAuthorNotch1 targeting siRNA-
dc.subject.keywordAuthorsiRNA delivery-
dc.subject.keywordAuthorNanoparticles-
dc.subject.keywordAuthorRheumatoid arthritis-
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