Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Min, Duyoung | - |
dc.contributor.author | Kim, Kipom | - |
dc.contributor.author | Hyeon, Changbong | - |
dc.contributor.author | Cho, Yong Hoon | - |
dc.contributor.author | Shin, Yeon-Kyun | - |
dc.contributor.author | Yoon, Tae-Young | - |
dc.date.accessioned | 2024-01-20T12:33:48Z | - |
dc.date.available | 2024-01-20T12:33:48Z | - |
dc.date.created | 2021-09-01 | - |
dc.date.issued | 2013-04 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | https://pubs.kist.re.kr/handle/201004/128217 | - |
dc.description.abstract | Formation of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex provides mechanical thrust for membrane fusion, but its molecular mechanism is still unclear. Here using magnetic tweezers, we observe mechanical responses of a single neuronal SNARE complex under constant pulling force. Single SNARE complexes may be unzipped with 34 pN force. When rezipping is induced by lowering the force to 11 pN, only a partially assembled state results, with the C-terminal half of the SNARE complex remaining disassembled. Reassembly of the C-terminal half occurs only when the force is further lowered below 11 pN. Thus, mechanical hysteresis, characterized by the unzipping and rezipping cycle of a single SNARE complex, produces the partially assembled state. In this metastable state, unzipping toward the N-terminus is suppressed while zippering toward the C-terminus is initiated as a steep function of force. This ensures the directionality of SNARE-complex formation, making the SNARE complex a robust force-generating machine. | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.subject | MAGNETIC TWEEZERS | - |
dc.subject | VESICLE FUSION | - |
dc.subject | SYNAPTOBREVIN | - |
dc.subject | DNA | - |
dc.subject | MICROMANIPULATION | - |
dc.subject | SYNAPTOTAGMIN-1 | - |
dc.subject | DYNAMICS | - |
dc.subject | BINDING | - |
dc.title | Mechanical unzipping and rezipping of a single SNARE complex reveals hysteresis as a force-generating mechanism | - |
dc.type | Article | - |
dc.identifier.doi | 10.1038/ncomms2692 | - |
dc.description.journalClass | 1 | - |
dc.identifier.bibliographicCitation | NATURE COMMUNICATIONS, v.4 | - |
dc.citation.title | NATURE COMMUNICATIONS | - |
dc.citation.volume | 4 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.identifier.wosid | 000318872100062 | - |
dc.identifier.scopusid | 2-s2.0-84877779303 | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | MAGNETIC TWEEZERS | - |
dc.subject.keywordPlus | VESICLE FUSION | - |
dc.subject.keywordPlus | SYNAPTOBREVIN | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | MICROMANIPULATION | - |
dc.subject.keywordPlus | SYNAPTOTAGMIN-1 | - |
dc.subject.keywordPlus | DYNAMICS | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordAuthor | Biological sciences | - |
dc.subject.keywordAuthor | Biophysics | - |
dc.subject.keywordAuthor | Cell biology | - |
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