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dc.contributor.authorMin, Duyoung-
dc.contributor.authorKim, Kipom-
dc.contributor.authorHyeon, Changbong-
dc.contributor.authorCho, Yong Hoon-
dc.contributor.authorShin, Yeon-Kyun-
dc.contributor.authorYoon, Tae-Young-
dc.date.accessioned2024-01-20T12:33:48Z-
dc.date.available2024-01-20T12:33:48Z-
dc.date.created2021-09-01-
dc.date.issued2013-04-
dc.identifier.issn2041-1723-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/128217-
dc.description.abstractFormation of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex provides mechanical thrust for membrane fusion, but its molecular mechanism is still unclear. Here using magnetic tweezers, we observe mechanical responses of a single neuronal SNARE complex under constant pulling force. Single SNARE complexes may be unzipped with 34 pN force. When rezipping is induced by lowering the force to 11 pN, only a partially assembled state results, with the C-terminal half of the SNARE complex remaining disassembled. Reassembly of the C-terminal half occurs only when the force is further lowered below 11 pN. Thus, mechanical hysteresis, characterized by the unzipping and rezipping cycle of a single SNARE complex, produces the partially assembled state. In this metastable state, unzipping toward the N-terminus is suppressed while zippering toward the C-terminus is initiated as a steep function of force. This ensures the directionality of SNARE-complex formation, making the SNARE complex a robust force-generating machine.-
dc.languageEnglish-
dc.publisherNATURE PUBLISHING GROUP-
dc.subjectMAGNETIC TWEEZERS-
dc.subjectVESICLE FUSION-
dc.subjectSYNAPTOBREVIN-
dc.subjectDNA-
dc.subjectMICROMANIPULATION-
dc.subjectSYNAPTOTAGMIN-1-
dc.subjectDYNAMICS-
dc.subjectBINDING-
dc.titleMechanical unzipping and rezipping of a single SNARE complex reveals hysteresis as a force-generating mechanism-
dc.typeArticle-
dc.identifier.doi10.1038/ncomms2692-
dc.description.journalClass1-
dc.identifier.bibliographicCitationNATURE COMMUNICATIONS, v.4-
dc.citation.titleNATURE COMMUNICATIONS-
dc.citation.volume4-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.identifier.wosid000318872100062-
dc.identifier.scopusid2-s2.0-84877779303-
dc.relation.journalWebOfScienceCategoryMultidisciplinary Sciences-
dc.relation.journalResearchAreaScience & Technology - Other Topics-
dc.type.docTypeArticle-
dc.subject.keywordPlusMAGNETIC TWEEZERS-
dc.subject.keywordPlusVESICLE FUSION-
dc.subject.keywordPlusSYNAPTOBREVIN-
dc.subject.keywordPlusDNA-
dc.subject.keywordPlusMICROMANIPULATION-
dc.subject.keywordPlusSYNAPTOTAGMIN-1-
dc.subject.keywordPlusDYNAMICS-
dc.subject.keywordPlusBINDING-
dc.subject.keywordAuthorBiological sciences-
dc.subject.keywordAuthorBiophysics-
dc.subject.keywordAuthorCell biology-
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KIST Article > 2013
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