Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Jeong, Kyuho | - |
dc.contributor.author | Kwon, Hayeong | - |
dc.contributor.author | Lee, Jaewoong | - |
dc.contributor.author | Jang, Donghwan | - |
dc.contributor.author | Hwang, Eun Mi | - |
dc.contributor.author | Park, Jae-Yong | - |
dc.contributor.author | Pak, Yunbae | - |
dc.date.accessioned | 2024-01-20T14:03:28Z | - |
dc.date.available | 2024-01-20T14:03:28Z | - |
dc.date.created | 2021-09-04 | - |
dc.date.issued | 2012-09 | - |
dc.identifier.issn | 1398-9219 | - |
dc.identifier.uri | https://pubs.kist.re.kr/handle/201004/128939 | - |
dc.description.abstract | Here, we have identified a retrograde transport pathway of caveolin-2 (cav-2) for its regulatory function in the nucleus. Confocal microscopy analysis, photoactivation experiments and subcellular fractionation revealed that cav-2 localized in the Golgi was transported to the inner nuclear membrane (INM) in response to insulin. Exogenous caveolin-1 (cav-1) and P132L-cav-1 expression did not affect the Golgi localization and insulin-induced INM targeting of cav-2. Cav-2DKV mutant in the endoplasmic reticulum (ER) was unable to translocate to the INM in response to insulin. The GTP-bound form of Rab6 promoted, but Rab6 siRNA and the GDP-bound form of Rab6 abrogated, retrograde trafficking of cav-2 from the Golgi to ER. Colchicine or nocodazole treatment abolished insulin-induced INM targeting of cav-2. Knock down of gp210 inhibited insulin-induced import of cav-2 from ER/outer nuclear membrane (ONM) to the INM. The INM-targeted cav-2 prevented heterochromatinization and promoted transcriptional activation of Elk-1 and signal transducer and activator of transcription 3 (STAT3). The results provide molecular mechanisms for insulin-induced INM translocation of cav-2 initiated (i) by Golgi-to-ER retrograde trafficking of cav-2 via microtubule-based Rab6-GTP-dependent transport and subsequently processed (ii) by gp210-mediated import of cav-2 from ER/ONM to INM. | - |
dc.language | English | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.subject | ENDOPLASMIC-RETICULUM | - |
dc.subject | GOLGI-COMPLEX | - |
dc.subject | GLUCOSE TRANSPORTERS | - |
dc.subject | ACTIN CYTOSKELETON | - |
dc.subject | PLASMA-MEMBRANE | - |
dc.subject | COATED VESICLES | - |
dc.subject | PORE COMPLEX | - |
dc.subject | LAMIN-A/C | - |
dc.subject | CELLS | - |
dc.subject | PROTEINS | - |
dc.title | Rab6-Mediated Retrograde Transport Regulates Inner Nuclear Membrane Targeting of Caveolin-2 in Response to Insulin | - |
dc.type | Article | - |
dc.identifier.doi | 10.1111/j.1600-0854.2012.01378.x | - |
dc.description.journalClass | 1 | - |
dc.identifier.bibliographicCitation | TRAFFIC, v.13, no.9, pp.1218 - 1233 | - |
dc.citation.title | TRAFFIC | - |
dc.citation.volume | 13 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1218 | - |
dc.citation.endPage | 1233 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.identifier.wosid | 000307724900004 | - |
dc.identifier.scopusid | 2-s2.0-84865178609 | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | ENDOPLASMIC-RETICULUM | - |
dc.subject.keywordPlus | GOLGI-COMPLEX | - |
dc.subject.keywordPlus | GLUCOSE TRANSPORTERS | - |
dc.subject.keywordPlus | ACTIN CYTOSKELETON | - |
dc.subject.keywordPlus | PLASMA-MEMBRANE | - |
dc.subject.keywordPlus | COATED VESICLES | - |
dc.subject.keywordPlus | PORE COMPLEX | - |
dc.subject.keywordPlus | LAMIN-A/C | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordAuthor | caveolin-2 | - |
dc.subject.keywordAuthor | gp210 | - |
dc.subject.keywordAuthor | heterochromatinization | - |
dc.subject.keywordAuthor | inner nuclear membrane | - |
dc.subject.keywordAuthor | insulin | - |
dc.subject.keywordAuthor | microtubules | - |
dc.subject.keywordAuthor | Rab6 | - |
dc.subject.keywordAuthor | retrograde transport | - |
dc.subject.keywordAuthor | transcriptional activation | - |
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