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dc.contributor.authorNam, Jeonghun-
dc.contributor.authorLim, Hyunjung-
dc.contributor.authorKim, Choong-
dc.contributor.authorKang, Ji Yoon-
dc.contributor.authorShin, Sehyun-
dc.date.accessioned2024-01-20T14:33:38Z-
dc.date.available2024-01-20T14:33:38Z-
dc.date.created2021-09-05-
dc.date.issued2012-06-
dc.identifier.issn1932-1058-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/129188-
dc.description.abstractThis study presents a method for density-based separation of monodisperse encapsulated cells using a standing surface acoustic wave (SSAW) in a microchannel. Even though monodisperse polymer beads can be generated by the state-of-the-art technology in microfluidics, the quantity of encapsulated cells cannot be controlled precisely. In the present study, mono-disperse alginate beads in a laminar flow can be separated based on their density using acoustophoresis. A mixture of beads of equal sizes but dissimilar densities was hydrodynamically focused at the entrance and then actively driven toward the sidewalls by a SSAW. The lateral displacement of a bead is proportional to the density of the bead, i.e., the number of encapsulated cells in an alginate bead. Under optimized conditions, the recovery rate of a target bead group (large-cell-quantity alginate beads) reached up to 97% at a rate of 2300 beads per minute. A cell viability test also confirmed that the encapsulated cells were hardly damaged by the acoustic force. Moreover, cell-encapsulating beads that were cultured for 1 day were separated in a similar manner. In conclusion, this study demonstrated that a SSAW can successfully separate monodisperse particles by their density. With the present technique for separating cell-encapsulating beads, the current cell engineering technology can be significantly advanced. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4718719]-
dc.languageEnglish-
dc.publisherAMER INST PHYSICS-
dc.subjectEMBRYONIC STEM-CELLS-
dc.subjectHYALURONIC-ACID-
dc.subjectEFFICIENT FORMATION-
dc.subjectSIZE-
dc.subjectHYDROGELS-
dc.subjectALGINATE-
dc.subjectBODIES-
dc.subjectDROPLETS-
dc.subjectTRANSPLANTATION-
dc.subjectEMULSIFICATION-
dc.titleDensity-dependent separation of encapsulated cells in a microfluidic channel by using a standing surface acoustic wave-
dc.typeArticle-
dc.identifier.doi10.1063/1.4718719-
dc.description.journalClass1-
dc.identifier.bibliographicCitationBIOMICROFLUIDICS, v.6, no.2-
dc.citation.titleBIOMICROFLUIDICS-
dc.citation.volume6-
dc.citation.number2-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.identifier.wosid000305839800031-
dc.identifier.scopusid2-s2.0-84863193401-
dc.relation.journalWebOfScienceCategoryBiochemical Research Methods-
dc.relation.journalWebOfScienceCategoryBiophysics-
dc.relation.journalWebOfScienceCategoryNanoscience & Nanotechnology-
dc.relation.journalWebOfScienceCategoryPhysics, Fluids & Plasmas-
dc.relation.journalResearchAreaBiochemistry & Molecular Biology-
dc.relation.journalResearchAreaBiophysics-
dc.relation.journalResearchAreaScience & Technology - Other Topics-
dc.relation.journalResearchAreaPhysics-
dc.type.docTypeArticle-
dc.subject.keywordPlusEMBRYONIC STEM-CELLS-
dc.subject.keywordPlusHYALURONIC-ACID-
dc.subject.keywordPlusEFFICIENT FORMATION-
dc.subject.keywordPlusSIZE-
dc.subject.keywordPlusHYDROGELS-
dc.subject.keywordPlusALGINATE-
dc.subject.keywordPlusBODIES-
dc.subject.keywordPlusDROPLETS-
dc.subject.keywordPlusTRANSPLANTATION-
dc.subject.keywordPlusEMULSIFICATION-
dc.subject.keywordAuthorbiological techniques-
dc.subject.keywordAuthorbioMEMS-
dc.subject.keywordAuthorcellular biophysics-
dc.subject.keywordAuthorflow separation-
dc.subject.keywordAuthorlaminar flow-
dc.subject.keywordAuthormicrochannel flow-
dc.subject.keywordAuthorsurface acoustic waves-
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