Full metadata record

DC Field Value Language
dc.contributor.authorKim, Jinhyun-
dc.contributor.authorZhao, Ting-
dc.contributor.authorPetralia, Ronald S.-
dc.contributor.authorYu, Yang-
dc.contributor.authorPeng, Hanchuan-
dc.contributor.authorMyers, Eugene-
dc.contributor.authorMagee, Jeffrey C.-
dc.date.accessioned2024-01-20T15:34:15Z-
dc.date.available2024-01-20T15:34:15Z-
dc.date.created2021-09-05-
dc.date.issued2012-01-
dc.identifier.issn1548-7091-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/129687-
dc.description.abstractThe GFP reconstitution across synaptic partners (GRASP) technique, based on functional complementation between two nonfluorescent GFP fragments, can be used to detect the location of synapses quickly, accurately and with high spatial resolution. The method has been previously applied in the nematode and the fruit fly but requires substantial modification for use in the mammalian brain. We developed mammalian GRASP (mGRASP) by optimizing transmembrane split-GFP carriers for mammalian synapses. Using in silico protein design, we engineered chimeric synaptic mGRASP fragments that were efficiently delivered to synaptic locations and reconstituted GFP fluorescence in vivo. Furthermore, by integrating molecular and cellular approaches with a computational strategy for the three-dimensional reconstruction of neurons, we applied mGRASP to both long-range circuits and local microcircuits in the mouse hippocampus and thalamocortical regions, analyzing synaptic distribution in single neurons and in dendritic compartments.-
dc.languageEnglish-
dc.publisherNATURE PUBLISHING GROUP-
dc.subjectEXPRESSION-
dc.subjectCIRCUIT-
dc.subjectBRAIN-
dc.subjectNEUREXINS-
dc.subjectSYNAPSES-
dc.subjectPROTEINS-
dc.subjectSINGLE-
dc.subjectARCHITECTURE-
dc.subjectMEMBRANE-
dc.titlemGRASP enables mapping mammalian synaptic connectivity with light microscopy-
dc.typeArticle-
dc.identifier.doi10.1038/NMETH.1784-
dc.description.journalClass1-
dc.identifier.bibliographicCitationNATURE METHODS, v.9, no.1, pp.96 - U139-
dc.citation.titleNATURE METHODS-
dc.citation.volume9-
dc.citation.number1-
dc.citation.startPage96-
dc.citation.endPageU139-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.identifier.wosid000298667000037-
dc.identifier.scopusid2-s2.0-84856418376-
dc.relation.journalWebOfScienceCategoryBiochemical Research Methods-
dc.relation.journalResearchAreaBiochemistry & Molecular Biology-
dc.type.docTypeArticle-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusCIRCUIT-
dc.subject.keywordPlusBRAIN-
dc.subject.keywordPlusNEUREXINS-
dc.subject.keywordPlusSYNAPSES-
dc.subject.keywordPlusPROTEINS-
dc.subject.keywordPlusSINGLE-
dc.subject.keywordPlusARCHITECTURE-
dc.subject.keywordPlusMEMBRANE-
Appears in Collections:
KIST Article > 2012
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE