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dc.contributor.authorChoung, Sunghun-
dc.contributor.authorChun, Myung-Suk-
dc.contributor.authorKim, Chongyoup-
dc.date.accessioned2024-01-20T16:04:51Z-
dc.date.available2024-01-20T16:04:51Z-
dc.date.created2021-09-05-
dc.date.issued2011-10-
dc.identifier.issn0374-4884-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/129952-
dc.description.abstractDirect single molecule tracking of a polyelectrolyte chain by using fluorescence microscopy has allowed both the assumptions and the predictions of relevant theories to be tested. The center-of-mass displacement is determined as a function of the time that elapses between images, where the radius of gyration can be estimated from a first moment of the image distribution. The translational self-diffusion for the molecule is an ensemble property of the mean square displacement (MSD) with lag time in each trajectory. Experimentally viable two-dimensional imaging of semiflexible polyelectrolyte was performed on a fluorescein-labeled xanthan chain in electroosmosis-driven uniform flow fields. The radius of gyration was almost constant under variations of the electroosmotic flow velocity determined by an externally applied electric field. We try to develop a correction of the MSD in flow field, taking into account the velocity fluctuations. Its advantage allows acquiring the linear fit for the MSD vs lag time, for a good estimate of the translational diffusion. Increasing behavior of the diffusion with increasing fluid velocity ensures a quadratic equation fit, which should connect with the convective effect. Our results exhibit a screening effect such that strong screening caused by a high ionic concentration leads to higher diffusion due to the compact chain conformation. Considering the uniform flow serves as a basis for understanding the behavior of individual polyelectrolyte chains under controlled fluidic flow in confined spaces.-
dc.languageEnglish-
dc.publisherKOREAN PHYSICAL SOC-
dc.subjectDNA-MOLECULES-
dc.subjectDYNAMICS-
dc.subjectDIFFUSION-
dc.subjectCONFORMATION-
dc.subjectNANOCHANNELS-
dc.subjectCONFINEMENT-
dc.subjectMICROSCOPY-
dc.subjectSIMULATION-
dc.subjectMOTION-
dc.titleSingle Molecule Tracking of a Semiflexible Polyelectrolyte Chain in Solvent Under Uniform Electroosmotic Flows-
dc.typeArticle-
dc.identifier.doi10.3938/jkps.59.2847-
dc.description.journalClass1-
dc.identifier.bibliographicCitationJOURNAL OF THE KOREAN PHYSICAL SOCIETY, v.59, no.4, pp.2847 - 2854-
dc.citation.titleJOURNAL OF THE KOREAN PHYSICAL SOCIETY-
dc.citation.volume59-
dc.citation.number4-
dc.citation.startPage2847-
dc.citation.endPage2854-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.description.journalRegisteredClasskci-
dc.identifier.kciidART001596128-
dc.identifier.wosid000295915500036-
dc.identifier.scopusid2-s2.0-80054889647-
dc.relation.journalWebOfScienceCategoryPhysics, Multidisciplinary-
dc.relation.journalResearchAreaPhysics-
dc.type.docTypeArticle-
dc.subject.keywordPlusDNA-MOLECULES-
dc.subject.keywordPlusDYNAMICS-
dc.subject.keywordPlusDIFFUSION-
dc.subject.keywordPlusCONFORMATION-
dc.subject.keywordPlusNANOCHANNELS-
dc.subject.keywordPlusCONFINEMENT-
dc.subject.keywordPlusMICROSCOPY-
dc.subject.keywordPlusSIMULATION-
dc.subject.keywordPlusMOTION-
dc.subject.keywordAuthorSingle molecule tracking-
dc.subject.keywordAuthorPolyelectrolyte-
dc.subject.keywordAuthorChain conformation-
dc.subject.keywordAuthorTranslational diffusion-
dc.subject.keywordAuthorUniform flow-
dc.subject.keywordAuthorRheology-
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KIST Article > 2011
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