Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jo, Nam Hyun | - |
dc.contributor.author | Kim, Jung Young | - |
dc.contributor.author | El-Gamal, Mohammed I. | - |
dc.contributor.author | Choi, Won-Kyoung | - |
dc.contributor.author | Park, Jin-Hun | - |
dc.contributor.author | Kim, Eun Jung | - |
dc.contributor.author | Cho, Jung-Hyuck | - |
dc.contributor.author | Ha, Hyun-Joon | - |
dc.contributor.author | Choi, Tae Hyun | - |
dc.contributor.author | Oh, Chang-Hyun | - |
dc.date.accessioned | 2024-01-20T17:33:48Z | - |
dc.date.available | 2024-01-20T17:33:48Z | - |
dc.date.created | 2022-01-10 | - |
dc.date.issued | 2011-02 | - |
dc.identifier.issn | 0362-4803 | - |
dc.identifier.uri | https://pubs.kist.re.kr/handle/201004/130659 | - |
dc.description.abstract | Synthesis, radiolabelling, and in vitro evaluation of a new I-125-labelled iodouracil hexitol nucleoside analogue are reported. The target compound was successfully synthesized by an iodination-destannylation method and then purified by reverse phase HPLC. The radiochemical purity of the product was >99% with decay-corrected yields of 48 +/- 3%. In vitro cellular uptake testing was carried out using MCA and MCA-tk cell lines for comparison of compound 1 with [F-18] FHBG. The newly synthesized compound 1 showed higher accumulation in herpex simplex virus type 1 thymidine kinase (HSV1-tk) gene expression cell line (MCA-tk cell line) than in the wild type MCA cell line compared with [18 F] FHBG. The MCA-tk to MCA cellular uptake ratio for compound 1 was higher than that of [F-18] FHBG from 2 h after incubation. The radioiodine-labelled compound 1 (I-125, t(1/2) = 59.37 days) has a longer physical half-life than F-18-(t(1/2) = 110 min) labelled FHBG. Radioiodine-labelled compound 1 could be used for monitoring gene expression for a long time. The selectivity for MCA-tk cell line makes compound 1 a promising imaging agent for HSV1-tk expression. | - |
dc.language | English | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.subject | THYMIDINE KINASE GENE | - |
dc.subject | PET IMAGING AGENT | - |
dc.subject | EXPRESSION | - |
dc.subject | THERAPY | - |
dc.subject | FIAU | - |
dc.subject | NUCLEOSIDES | - |
dc.subject | ANALOGS | - |
dc.subject | PROBES | - |
dc.subject | SUGAR | - |
dc.subject | FHPG | - |
dc.title | Radiosynthesis and in vitro evaluation of 1-(tetrahydro-5-hydroxy-6-(hydroxymethyl)-2H-pyran-3-yl)-5-[I-125]iodour acil: A new potential agent for HSV1-tk reporter gene monitoring | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/jlcr.1819 | - |
dc.description.journalClass | 1 | - |
dc.identifier.bibliographicCitation | JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS, v.54, no.2, pp.93 - 97 | - |
dc.citation.title | JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS | - |
dc.citation.volume | 54 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 93 | - |
dc.citation.endPage | 97 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.identifier.wosid | 000287612500007 | - |
dc.identifier.scopusid | 2-s2.0-79951733519 | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | THYMIDINE KINASE GENE | - |
dc.subject.keywordPlus | PET IMAGING AGENT | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | FIAU | - |
dc.subject.keywordPlus | NUCLEOSIDES | - |
dc.subject.keywordPlus | ANALOGS | - |
dc.subject.keywordPlus | PROBES | - |
dc.subject.keywordPlus | SUGAR | - |
dc.subject.keywordPlus | FHPG | - |
dc.subject.keywordAuthor | HSV1-tk | - |
dc.subject.keywordAuthor | nucleoside | - |
dc.subject.keywordAuthor | hexitol | - |
dc.subject.keywordAuthor | iodouracil | - |
dc.subject.keywordAuthor | MCA-tk | - |
dc.subject.keywordAuthor | MCA | - |
dc.subject.keywordAuthor | iodine-125 | - |
dc.subject.keywordAuthor | imaging agents | - |
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