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dc.contributor.authorKim, Hea Kyung-
dc.contributor.authorShim, Woo Sun-
dc.contributor.authorKim, Sung Eun-
dc.contributor.authorLee, Kweon-Haeng-
dc.contributor.authorKang, Eunah-
dc.contributor.authorKim, Jong-Ho-
dc.contributor.authorKim, Kwangmeyung-
dc.contributor.authorKwon, Ick Chan-
dc.contributor.authorLee, Doo Sung-
dc.date.accessioned2024-01-20T21:34:28Z-
dc.date.available2024-01-20T21:34:28Z-
dc.date.created2021-09-03-
dc.date.issued2009-04-
dc.identifier.issn1937-3341-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/132617-
dc.description.abstractWe developed a novel pH- and thermo-sensitive hydrogel as a scaffold for autologous bone tissue engineering. We synthesized this polymer by adding pH- sensitive sulfamethazine oligomers (SMOs) to both ends of a thermo-sensitive poly(epsilon-caprolactone-co-lactide)-poly(ethylene glycol)-poly(e-caprolactone-co-lactide) (PCLA-PEG-PCLA) block copolymer, yielding a pH/thermo-sensitive SMO-PCLA-PEG-PCLA-SMO block copolymer. The synthesized block copolymer solution rapidly formed a stable gel under physiological conditions (pH 7.4 and 37 degrees C), whereas it formed a sol at pH 8.0 and 37 degrees C, making it injectable. This pH/thermo-sensitive hydrogel exhibited high biocompatibility in a Dulbecco's modified Eagle's medium extract test. Under physiological conditions, the hydrogel easily encapsulated human mesenchymal stem cells (hMSCs) and recombinant human bone morphogenetic protein-2 (rhBMP-2), with encapsulating efficiencies of about 90% and 85%, respectively. To assay for ectopic bone formation in vivo, we subcutaneously injected a polymer solution containing hMSCs and rhBMP-2 into the back of mice, after which we could observe hMSC differentiation for up to 7 weeks. Histological studies revealed mineralized tissue formation and high levels of alkaline phosphatase activity in the mineralized tissue. Therefore, this pH/thermo-sensitive SMO-PCLA-PEG-PCLA-SMO block copolymer demonstrated potential as an injectable scaffold for bone tissue engineering, with in situ formation capabilities.-
dc.languageEnglish-
dc.publisherMARY ANN LIEBERT, INC-
dc.subjectMESENCHYMAL STEM-CELLS-
dc.subjectMORPHOGENETIC PROTEIN-2-
dc.subjectADIPOSE-TISSUE-
dc.subjectOSTEOGENIC DIFFERENTIATION-
dc.subjectDELIVERY-
dc.subjectPH-
dc.subjectDEGRADATION-
dc.subjectSCAFFOLDS-
dc.subjectMATRICES-
dc.titleInjectable In Situ-Forming pH/Thermo-Sensitive Hydrogel for Bone Tissue Engineering-
dc.typeArticle-
dc.identifier.doi10.1089/ten.tea.2007.0407-
dc.description.journalClass1-
dc.identifier.bibliographicCitationTISSUE ENGINEERING PART A, v.15, no.4, pp.923 - 933-
dc.citation.titleTISSUE ENGINEERING PART A-
dc.citation.volume15-
dc.citation.number4-
dc.citation.startPage923-
dc.citation.endPage933-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.identifier.wosid000264892000019-
dc.identifier.scopusid2-s2.0-67049162753-
dc.relation.journalWebOfScienceCategoryCell & Tissue Engineering-
dc.relation.journalWebOfScienceCategoryCell Biology-
dc.relation.journalWebOfScienceCategoryEngineering, Biomedical-
dc.relation.journalWebOfScienceCategoryMaterials Science, Biomaterials-
dc.relation.journalResearchAreaCell Biology-
dc.relation.journalResearchAreaEngineering-
dc.relation.journalResearchAreaMaterials Science-
dc.type.docTypeArticle-
dc.subject.keywordPlusMESENCHYMAL STEM-CELLS-
dc.subject.keywordPlusMORPHOGENETIC PROTEIN-2-
dc.subject.keywordPlusADIPOSE-TISSUE-
dc.subject.keywordPlusOSTEOGENIC DIFFERENTIATION-
dc.subject.keywordPlusDELIVERY-
dc.subject.keywordPlusPH-
dc.subject.keywordPlusDEGRADATION-
dc.subject.keywordPlusSCAFFOLDS-
dc.subject.keywordPlusMATRICES-
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KIST Article > 2009
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