Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Kim, Hea Kyung | - |
dc.contributor.author | Shim, Woo Sun | - |
dc.contributor.author | Kim, Sung Eun | - |
dc.contributor.author | Lee, Kweon-Haeng | - |
dc.contributor.author | Kang, Eunah | - |
dc.contributor.author | Kim, Jong-Ho | - |
dc.contributor.author | Kim, Kwangmeyung | - |
dc.contributor.author | Kwon, Ick Chan | - |
dc.contributor.author | Lee, Doo Sung | - |
dc.date.accessioned | 2024-01-20T21:34:28Z | - |
dc.date.available | 2024-01-20T21:34:28Z | - |
dc.date.created | 2021-09-03 | - |
dc.date.issued | 2009-04 | - |
dc.identifier.issn | 1937-3341 | - |
dc.identifier.uri | https://pubs.kist.re.kr/handle/201004/132617 | - |
dc.description.abstract | We developed a novel pH- and thermo-sensitive hydrogel as a scaffold for autologous bone tissue engineering. We synthesized this polymer by adding pH- sensitive sulfamethazine oligomers (SMOs) to both ends of a thermo-sensitive poly(epsilon-caprolactone-co-lactide)-poly(ethylene glycol)-poly(e-caprolactone-co-lactide) (PCLA-PEG-PCLA) block copolymer, yielding a pH/thermo-sensitive SMO-PCLA-PEG-PCLA-SMO block copolymer. The synthesized block copolymer solution rapidly formed a stable gel under physiological conditions (pH 7.4 and 37 degrees C), whereas it formed a sol at pH 8.0 and 37 degrees C, making it injectable. This pH/thermo-sensitive hydrogel exhibited high biocompatibility in a Dulbecco's modified Eagle's medium extract test. Under physiological conditions, the hydrogel easily encapsulated human mesenchymal stem cells (hMSCs) and recombinant human bone morphogenetic protein-2 (rhBMP-2), with encapsulating efficiencies of about 90% and 85%, respectively. To assay for ectopic bone formation in vivo, we subcutaneously injected a polymer solution containing hMSCs and rhBMP-2 into the back of mice, after which we could observe hMSC differentiation for up to 7 weeks. Histological studies revealed mineralized tissue formation and high levels of alkaline phosphatase activity in the mineralized tissue. Therefore, this pH/thermo-sensitive SMO-PCLA-PEG-PCLA-SMO block copolymer demonstrated potential as an injectable scaffold for bone tissue engineering, with in situ formation capabilities. | - |
dc.language | English | - |
dc.publisher | MARY ANN LIEBERT, INC | - |
dc.subject | MESENCHYMAL STEM-CELLS | - |
dc.subject | MORPHOGENETIC PROTEIN-2 | - |
dc.subject | ADIPOSE-TISSUE | - |
dc.subject | OSTEOGENIC DIFFERENTIATION | - |
dc.subject | DELIVERY | - |
dc.subject | PH | - |
dc.subject | DEGRADATION | - |
dc.subject | SCAFFOLDS | - |
dc.subject | MATRICES | - |
dc.title | Injectable In Situ-Forming pH/Thermo-Sensitive Hydrogel for Bone Tissue Engineering | - |
dc.type | Article | - |
dc.identifier.doi | 10.1089/ten.tea.2007.0407 | - |
dc.description.journalClass | 1 | - |
dc.identifier.bibliographicCitation | TISSUE ENGINEERING PART A, v.15, no.4, pp.923 - 933 | - |
dc.citation.title | TISSUE ENGINEERING PART A | - |
dc.citation.volume | 15 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 923 | - |
dc.citation.endPage | 933 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.identifier.wosid | 000264892000019 | - |
dc.identifier.scopusid | 2-s2.0-67049162753 | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | MESENCHYMAL STEM-CELLS | - |
dc.subject.keywordPlus | MORPHOGENETIC PROTEIN-2 | - |
dc.subject.keywordPlus | ADIPOSE-TISSUE | - |
dc.subject.keywordPlus | OSTEOGENIC DIFFERENTIATION | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | PH | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | SCAFFOLDS | - |
dc.subject.keywordPlus | MATRICES | - |
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