C18-PEG로 화학흡착된 실리콘 웨이퍼의 표면특성 및 선택적 알부민 흡착

Abstract

Adsorption of albumin to biomaterial surfaces has been useful in both medical and pharmaceutical applications. It is known that the affinity of albumin increases by grafting C18 alkyl chain to the biomaterial surfaces. Brij® (alkyl PEGOH), which has both long poly(ethylene glycol) (PEG) side chain and stearyl (C18) alkyl end group, is specifically designed to get the functional surface to trigger the adsorption of albumin alone. In this study, thiol-derived Brij® (alkyl PEG-SH) macromer was synthesized from substituting terminal hydroxyl group of Brij® through tosylation, and the alkyl PEG-SH was assembled and chemisorbed on the gold-coated silicon wafer. The chemisorbed surface was characterized using ATR-FTIR, ESCA, SEM, and contact angles and albumin adsorption test. Since the Si surface modified with C18-PEG20 has a highly selective affinity to albumin, it can be useful as a nano-scale biosensor for protein chip, because it has mobile PEG groups for non-specific protein repulsion and C18 alkyl chains for selective adsorption of albumin.