The rapid identification of isoflavonoids from Belamcanda chinensis by LC-NMR and LG-MS

Abstract

The use of hyphenated LC-NMR and LC-MS techniques for the purpose of directly identifying the major constituents of Belamcanda chinensis was investigated. Reversed-phase isocratic chromatography performed using an acetonitrile-water solvent system on a C18 column. The NMR spectrum yielded five main peaks, whose analysis revealed them to he 5, 6, 7, 3'-tetrahydroxy-4'-methoxyisoflavone (1), tectorioenin (2), iristectorigenin A (3), irigenin (4), and irisflorentine (5). The identification of these constituents was confirmed by performing LC-ESI-MS experiment. This study shows that hyphenated LC-NMR and LC-MS can he used for the rapid (70 min) identification of the isoflavonoids.