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dc.contributor.authorKim, SK-
dc.contributor.authorKim, K-
dc.contributor.authorLee, S-
dc.contributor.authorPark, K-
dc.contributor.authorPark, JH-
dc.contributor.authorKwon, IC-
dc.contributor.authorChoi, K-
dc.contributor.authorKim, CY-
dc.contributor.authorByun, Y-
dc.date.accessioned2024-01-21T04:10:26Z-
dc.date.available2024-01-21T04:10:26Z-
dc.date.created2021-09-03-
dc.date.issued2005-10-04-
dc.identifier.issn0731-7085-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/136058-
dc.description.abstractWe validated the application of the surface plasmon resonance (SPR) technique to reliably determine adhesion of drugs to the intestinal wall using heparin-DOCA conjugates, developed to enhance the oral absorption of poorly absorbed heparin. In this study, heparin conjugates, or deoxycholyl-heparin (H-DOCA) and bisdeoxycholyl-heparin (H-bis-DOCA), were synthesized by covalently coupling the synthesized succinimido deoxycholate (DOCA-NHS) or succinimido bis-deoxycholyl-L-lysine (DOCA-bis-NHS) to amine groups of heparin, and their physicochemical and biological properties were evaluated. To mimic the duodenal and ileal surfaces, duodenal and ileal brush border membrane (BBM) vesicles isolated from Sprauge-Dawley (SD) rats were immobilized onto a biosensor chip composed of dextran derivatives with modified lipophilic residues. The adhesion of heparin conjugates on the BBM surface was evaluated by measuring the SPR response signal. The adhesion of heparin conjugates was significantly dependent on the conjugated DOCA molecules: that is, they showed higher adhesion signal on the ilea] BBM surface than that on the duodenal BBM surface. In particular, the solubilized heparin conjugates in DMSO solution presented significantly increased adhesion affinity on the ileal 1313M surface. The adhesion of heparin conjugates on the intestinal surfaces was successfully assayed using the surface plasmon resonance technique with the sensor chip on which BBM vesicles were immobilized. (c) 2005 Elsevier B.V. All rights reserved.-
dc.languageEnglish-
dc.publisherELSEVIER-
dc.subjectMOLECULAR-WEIGHT HEPARIN-
dc.subjectBRUSH-BORDER MEMBRANES-
dc.subjectBIOSENSOR-
dc.subjectDERIVATIVES-
dc.titleEvaluation of absorption of heparin-DOCA conjugates on the intestinal wall using a surface plasmon resonance-
dc.typeArticle-
dc.identifier.doi10.1016/j.jpba.2005.06.014-
dc.description.journalClass1-
dc.identifier.bibliographicCitationJOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, v.39, no.5, pp.861 - 870-
dc.citation.titleJOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS-
dc.citation.volume39-
dc.citation.number5-
dc.citation.startPage861-
dc.citation.endPage870-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.identifier.wosid000233067000002-
dc.identifier.scopusid2-s2.0-26644447763-
dc.relation.journalWebOfScienceCategoryChemistry, Analytical-
dc.relation.journalWebOfScienceCategoryPharmacology & Pharmacy-
dc.relation.journalResearchAreaChemistry-
dc.relation.journalResearchAreaPharmacology & Pharmacy-
dc.type.docTypeArticle-
dc.subject.keywordPlusMOLECULAR-WEIGHT HEPARIN-
dc.subject.keywordPlusBRUSH-BORDER MEMBRANES-
dc.subject.keywordPlusBIOSENSOR-
dc.subject.keywordPlusDERIVATIVES-
dc.subject.keywordAuthorheparin-
dc.subject.keywordAuthordeoxycholic acid-
dc.subject.keywordAuthorsurface plasmon resonance-
dc.subject.keywordAuthorbrush border membrane-
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