Full metadata record

DC Field Value Language
dc.contributor.authorKim, Keon Woo-
dc.contributor.authorKim, Dain-
dc.contributor.authorKim, Byoung Chan-
dc.contributor.authorHwang, Ee Taek-
dc.date.accessioned2024-08-01T06:00:12Z-
dc.date.available2024-08-01T06:00:12Z-
dc.date.created2024-08-01-
dc.date.issued2024-08-
dc.identifier.issn0141-8130-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/150339-
dc.description.abstractThe demand for glucose-sensing devices has increased along with the increasing diabetic population. Here, we aimed to construct a system with a glucose oxidase (GOx)-integrated Cu-nanoflower (Cu-NF) as the underlying electrode. This novel system was successfully developed by creating a cross-linked GOx within a Cu-NF matrix, forming a c-GOx@Cu-NF-coated film on a carbon screen-printed electrode (CSPE). A comparison of the stabilities of the cross-linking methods demonstrated enhanced durability, with an activity level of >88 % maintained after approximately 35 days of storage in room temperature buffer. Regarding the ability of the c-GOx@Cu-NF modified CSPE to detect glucose via electrochemical methods, the redox potential gap (Delta E) and peak current increased in the presence of GOx. In comparison to that of glucose, the sensitivity of c-GOx@Cu-NF was approximately 8 times greater than that of GOx@Cu-NF, with a detection limit of 0.649 mu M and a linear range of 5 - 500 mu M. It sustained an average relative activity of 80 % over 20 days. After 10 cycles of repeated use, the activity remained above 75 %. In terms of evaluating the electrode ' s specificity for glucose, the detection rate for individual similar substances was approximately 1 %. The introduction of a crosslinking strategy to Cu-NF, leading to enhanced mechanical stability and conductivity, improved the detection capability. Furthermore, this approach led to increased long-term storage stability and reusability, allowing for specific glucose detection. To our knowledge, this report represents the first demonstration of a c-GOx@Cu-NF system for integrating electrochemical biosensing devices into digital healthcare pathways, offering enhanced sensing accuracy and mechanical stability.-
dc.languageEnglish-
dc.publisherElsevier BV-
dc.titleDevelopment of cross-linked glucose oxidase integrated Cu-nanoflower electrode for reusable and stable glucose sensing-
dc.typeArticle-
dc.identifier.doi10.1016/j.ijbiomac.2024.133605-
dc.description.journalClass1-
dc.identifier.bibliographicCitationInternational Journal of Biological Macromolecules, v.275-
dc.citation.titleInternational Journal of Biological Macromolecules-
dc.citation.volume275-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.identifier.wosid001272842600001-
dc.identifier.scopusid2-s2.0-85198038889-
dc.relation.journalWebOfScienceCategoryBiochemistry & Molecular Biology-
dc.relation.journalWebOfScienceCategoryChemistry, Applied-
dc.relation.journalWebOfScienceCategoryPolymer Science-
dc.relation.journalResearchAreaBiochemistry & Molecular Biology-
dc.relation.journalResearchAreaChemistry-
dc.relation.journalResearchAreaPolymer Science-
dc.type.docTypeArticle-
dc.subject.keywordPlusMETAL-ORGANIC FRAMEWORKS-
dc.subject.keywordPlusELECTROCHEMICAL BIOSENSORS-
dc.subject.keywordPlusIMMOBILIZATION TECHNIQUES-
dc.subject.keywordPlusENZYME IMMOBILIZATION-
dc.subject.keywordPlusSTABILIZATION-
dc.subject.keywordPlusPRINCIPLES-
dc.subject.keywordPlusSTABILITY-
dc.subject.keywordPlusSENSOR-
dc.subject.keywordAuthorBiosensors-
dc.subject.keywordAuthorElectrochemical enzyme biosensor-
dc.subject.keywordAuthorCopper nanoflower-
dc.subject.keywordAuthorGlucose oxidase-
dc.subject.keywordAuthorReusable glucose electrode-
Appears in Collections:
KIST Article > 2024
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE